Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 338

The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.

A mechanism of proton translocation by F1F0 ATP synthases suggested by double mutants of the a subunit.

Open access
Vik, S B, Antonio, B J·1994·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)43822-7
Open this paper in CoChat — free account

Abstract

Three amino acid residues in the a subunit of the Escherichia coli F1F0 ATP synthase are essential for proton translocation: Arg210, Glu219, and His245. In this study, the essential glutamic acid has been relocated to position 252 with retention of function. It had been known that Gln252 can be replaced by Glu without significant effect. To test whether Q252E would function in the absence of Glu219, a "site-directed second-site suppressor" experiment was designed. Saturation mutagenesis was applied to residue Glu219, and 14 different amino acid substitutions were isolated, five of which permitted growth on succinate minimal medium at 37 degrees C: Asp, Lys, Gly, Ala, and Ser. These results indicate that Q252E can provide the essential carboxyl group normally provided by Glu219, but that strict requirements are placed on the residue at position 219. We interpret these results to mean that the Q252E must occupy, at least partially, the normal position of Glu219. We present a novel mechanism of proton translocation by F1F0 ATP synthases that includes a rotating oligomer of c subunits, in which the Asp61 of two c subunits simultaneously interact with Glu219 and Arg210 of the a subunit. This mechanism can be adapted for both mitochondrial and sodium-driven bacterial ATP synthases.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(18)43822-7

Cites

48 of 48 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 48 references

Cited by

See all 338

Metadata

Authors
Vik, S. B., & Antonio, B. J.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1994-12
Volume
269
Issue
48
Pages
30364-30369
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(18)43822-7
OpenAlex
W1592768266
Cited by
338
References
48
FWCI
3.43
Type
journal article
Language
en
Open access
hybrid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: A mechanism of proton translocation by F1F0 ATP synthases suggested by double mutants of the a subunit. https://app.cochat.ai/papers/10.1016/s0021-9258(18)43822-7
The paper
Vik, S. B., & Antonio, B. J. (1994). A mechanism of proton translocation by F1F0 ATP synthases suggested by double mutants of the a subunit. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)43822-7

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.