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Quantitative analysis of the human alpha-skeletal actin gene in transgenic mice.
Open accessAbstract
Three aspects of the regulation of the human alpha-skeletal actin gene are examined in this study by quantitative analysis of transgenic tissues: level of expression, tissue specificity, and developmental regulation. Previous in vitro and in vivo studies analyzing the 5' end of the gene have indicated that regulation of tissue-specific expression is promoter based. Transgenic mice were produced carrying either a 9.5-kilobase pair (kb) human alpha-skeletal actin gene fragment or a deletion construct with 2.2-kb of 5' sequences of human alpha-skeletal actin linked to the chloramphenicol acetyltransferase reporter gene. We found that the 9.5-kb transgene was capable of expression in adult skeletal muscle at a level equivalent to that of the endogenous gene in a non-transgenic mouse. The deletion construct was also capable of high-level expression. Both transgenes were expressed in a striated muscle-specific manner and were correctly regulated during development. We conclude that these three parameters of regulation of the human alpha-skeletal actin gene are mediated by sequences within the region -2000 to +239 of the promoter.
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Cited by
Bodine, S. C., Stitt, T. N., Gonzalez, M., Kline, W. O., Stover, G. L., Bauerlein, R., Zlotchenko, E., Scrimgeour, A., Lawrence, J. C., Glass, D. J., et al. (2001) · Nature Cell Biology · doi:10.1038/ncb1101-1014 · Cited by 2,752
Regulation of Muscle Fiber Type and Running Endurance by PPARδ
Wang, Y.-X., Zhang, C.-L., Yu, R. T., Cho, H. K., Nelson, M. C., Bayuga-Ocampo, C. R., Ham, J., Kang, H., & Evans, R. M. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020294 · Cited by 1,089
Coleman, M. E., DeMayo, F., Yin, K. C., Lee, H. M., Geske, R., Montgomery, C., & Schwartz, R. J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.20.12109 · Cited by 642
Mice overexpressing human uncoupling protein-3 in skeletal muscle are hyperphagic and lean
Clapham, J. C., Arch, J. R. S., Chapman, H., Haynes, A., Lister, C., Moore, G. B. T., Piercy, V., Carter, S. A., Lehner, I., Smith, S. A., et al. (2000) · Nature · doi:10.1038/35019082 · Cited by 604
Expression of full-length utrophin prevents muscular dystrophy in mdx mice
Tinsley, J., Deconinck, N., Fisher, R., Kahn, D., Phelps, S., Gillis, J.-M., & Davies, K. (1998) · Nature Medicine · doi:10.1038/4033 · Cited by 615
Metadata
- Authors
- Brennan, K. J., & Hardeman, E. C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-01
- Volume
- 268
- Issue
- 1
- Pages
- 719-725
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1514486148
- Cited by
- 167
- References
- 0
- FWCI
- 1.37
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Brennan, K. J., & Hardeman, E. C. (1993). Quantitative analysis of the human alpha-skeletal actin gene in transgenic mice. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)54211-3
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