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Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2.
Open accessAbstract
A general transcription factor (BTF2) has been purified from HeLa whole cell extract and shown to be absolutely required for the formation of a functional initiation complex. We also demonstrate that this factor binds in solution to RNA polymerase B(II) and to the other general transcription factors BTF1(TFIID), BTF3, and BTF4. These results strongly suggest the existence of multiple interactions between the various components of the multiprotein initiation complex.
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Citations
Cited by
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de Laat, W. L., Jaspers, N. G. J., & Hoeijmakers, J. H. J. (1999) · Genes & Development · doi:10.1101/gad.13.7.768 · Cited by 1,122
Mammalian DNA nucleotide excision repair reconstituted with purified protein components
Aboussekhra, A., Biggerstaff, M., Shivji, M. K. K., Vilpo, J. A., Moncollin, V., Podust, V. N., Protić, M., Hübscher, U., Egly, J.-M., & Wood, R. D. (1995) · Cell · doi:10.1016/0092-8674(95)90289-9 · Cited by 837
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de Boer, J., & Hoeijmakers, J. H. J. (2000) · Carcinogenesis · doi:10.1093/carcin/21.3.453 · Cited by 674
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Gillet, L. C. J., & Schärer, O. D. (2006) · Chemical Reviews · doi:10.1021/cr040483f · Cited by 642
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Metadata
- Authors
- Gerard, M., Fischer, L., Moncollin, V., Chipoulet, J. M., Chambon, P., & Egly, J. M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1991-11
- Volume
- 266
- Issue
- 31
- Pages
- 20940-20945
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1606394252
- Cited by
- 200
- References
- 0
- FWCI
- 4.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2. https://app.cochat.ai/papers/10.1016/s0021-9258(18)54801-8
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- Gerard, M., Fischer, L., Moncollin, V., Chipoulet, J. M., Chambon, P., & Egly, J. M. (1991). Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)54801-8
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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