The DOI matches its Crossref record.
A QUANTITATIVE COLOR REACTION FOR CORTISONE AND RELATED 17,21-DIHYDROXY-20-KETOSTEROIDS
Open accessAbstract
The numerous combinations of functional groups occurring in the steroid hormones render difficult the development of a specific test for individual compounds. However, reactions which reveal certain groupings are available and may be applied singly or in combination to elucidate the characteristics of pure steroids or of compounds in a mixture. The ketol side chain, located at carbon atom 17 in certain corticosteroids, has reducing properties and also yields formaldehyde upon oxidation with periodic acid. These reactions have been applied in the analysis of partially purified steroid mixtures (14). The reaction of active carbonyl groups in steroids with phenylhydrazines is the basis of histochemical methods (5). Production of colored and fluorescent solutions in sulfuric acid (6) and reaction with antimony trichloride (7), dinitrobenzene (8), and other reagents (9) have also been employed. To this group may now be added another test which appears to be specific for 17,21-dihydroxy-20-ketosteroids. The method is based upon the appearance of a yellow color in solutions containing the steroid, phenylhydrazine, and sulfuric acid.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Cushing's syndrome: a review of diagnostic tests.
Crapo, L. (1979) · Metabolism · doi:10.1016/0026-0495(79)90097-0 · Cited by 393
Evaluation of Silber-Porter Procedure for Determination of Plasma Hydrocortisone
Peterson, R. E., Karrer, A., & Guerra, S. L. (1957) · Analytical Chemistry · doi:10.1021/ac60121a042 · Cited by 391
The adrenogenital syndrome with deficiency of 3β-hydroxysteroid dehydrogenase
Bongiovanni, A. M. (1962) · Journal of Clinical Investigation · doi:10.1172/jci104666 · Cited by 304
Use of ACTH in the diagnosis of adrenal cortical insufficiency
Jenkins, D., Forsham, P. H., Laidlaw, J. C., Reddy, W. J., & Thorn, G. W. (1955) · The American Journal of Medicine · doi:10.1016/0002-9343(55)90200-x · Cited by 127
High fluid intake increases urine free cortisol excretion in normal subjects.
Mericq, M. V., & Cutler, G. B. (1998) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.83.2.4555 · Cited by 111
Metadata
- Authors
- Porter, C. C., & Silber, R. H.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1950-07
- Volume
- 185
- Issue
- 1
- Pages
- 201-207
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W991319
- Cited by
- 563
- References
- 0
- FWCI
- 16.31
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A QUANTITATIVE COLOR REACTION FOR CORTISONE AND RELATED 17,21-DIHYDROXY-20-KETOSTEROIDS. https://app.cochat.ai/papers/10.1016/s0021-9258(18)56408-5
- The paper
- Porter, C. C., & Silber, R. H. (1950). A QUANTITATIVE COLOR REACTION FOR CORTISONE AND RELATED 17,21-DIHYDROXY-20-KETOSTEROIDS. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)56408-5
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
