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Cited by 605

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Reconstitution of Troponin Activity from Three Protein Components

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Greaser, M.L., Gergely, J.·1971·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)62075-7
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Abstract

Pursed troponin prepared according to published procedures was separated into four major protein fractions when chromatographed on DEAE-Sephadex in 6 rd urea.The main components in Fractions 1 to 4 had approximate molecular weights of 14,000,24,000,35,000, and 21,000, respectively, as determined by sodium dodecyl sulfate gel electrophoresis.Reconstitution experiments using all possible combinations indicated that Fractions 2, 3, and 4 restored the Ca+f requirement for the ATPase activity of actomyosin in the presence of tropomyosin.Recovery of activity could only be achieved when the required components were mixed prior to removal of urea.This suggests that one or more of these proteins may refold improperly on removal of urea unless the other components are present.

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Metadata

Authors
Greaser, M. L., & Gergely, J.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1971-07
Volume
246
Issue
13
Pages
4226-4233
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(18)62075-7
OpenAlex
W1538939790
Cited by
605
References
0
FWCI
31.82
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Reconstitution of Troponin Activity from Three Protein Components. https://app.cochat.ai/papers/10.1016/s0021-9258(18)62075-7
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Greaser, M. L., & Gergely, J. (1971). Reconstitution of Troponin Activity from Three Protein Components. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)62075-7

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.