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Molecular Cloning of Human Intestinal Mucin cDNAs
Open accessAbstract
A human small intestine lambda gt11 cDNA library was screened using antisera prepared against the deglycosylated protein backbone of human colon cancer xenograft mucin. Three cDNAs were isolated from this screening, designated SMUC 40-42. These cDNAs were all found to contain tandem repeats of 69 nucleotides which encoded a threonine- and proline-rich protein consensus sequence of PTTTPITTTTTVTPTPTPTGTQT. RNA blots probed with one of these cDNAs, SMUC 41, exhibited large, polydisperse hybridization bands at approximately 7,600 bases. Band intensities were strongest when human small intestine, colon, and colon cancer poly(A)+ RNA was used. In vitro translation of poly(A)+ RNA from human small intestine, colon, and colon cancer cells produced a 162,000-dalton peptide that was immunoprecipitated with antibodies to deglycosylated mucin. SMUC 41 was also used to probe DNA blots, which indicated the presence of restriction fragment length polymorphisms in the intestinal mucin gene. These findings may be important in assessing the abnormal mucins found associated with several human diseases.
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Cited by
The gastrointestinal mucus system in health and disease
Johansson, M. E. V., Sjövall, H., & Hansson, G. C. (2013) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2013.35 · Cited by 1,438
Intestinal barrier dysfunction in inflammatory bowel diseases
McGuckin, M. A., Eri, R., Simms, L. A., Florin, T. H. J., & Radford-Smith, G. (2009) · Inflammatory Bowel Diseases · doi:10.1002/ibd.20539 · Cited by 627
Mucin-bacterial interactions in the human oral cavity and digestive tract
Derrien, M., van Passel, M. W. J., van de Bovenkamp, J. H. B., Schipper, R., de Vos, W., & Dekker, J. (2010) · Gut Microbes · doi:10.4161/gmic.1.4.12778 · Cited by 556
Mucins and mucin binding proteins in colorectal cancer
Byrd, J. C., & Bresalier, R. S. (2004) · Cancer and Metastasis Reviews · doi:10.1023/a:1025815113599 · Cited by 490
Self-citationBobek, L. A., Tsai, H., Biesbrock, A. R., & Levine, M. J. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)80762-5 · Cited by 458
Metadata
- Authors
- Gum, J. R., Byrd, J. C., Hicks, J. W., Toribara, N. W., Lamport, D. T. A., & Kim, Y. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1989-04
- Volume
- 264
- Issue
- 11
- Pages
- 6480-6487
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1548863075
- Cited by
- 541
- References
- 0
- FWCI
- 13.02
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Gum, J. R., Byrd, J. C., Hicks, J. W., Toribara, N. W., Lamport, D. T. A., & Kim, Y. S. (1989). Molecular Cloning of Human Intestinal Mucin cDNAs. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)83373-7
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