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Cited by 332

The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.

Interaction of a 59-kDa Connective Tissue Matrix Protein with Collagen I and Collagen II

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Hedbom, E, Heinegård, D·1989·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)83516-5
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Abstract

We have studied binding to collagen of the 59-kDa protein present in most connective tissues. Collagen fibril formation, measured as increasing turbidity, was markedly retarded and reduced by the presence of small amounts of this protein. This was true for both collagen I and collagen II. The effect was also observed when pepsin-treated collagens were used, indicating that interaction with the telopeptides is not involved. The proportion of collagen precipitated in the assay was not or only marginally reduced. Thus, the altered optical properties indicate that structurally different fibrils are formed in the presence of the 59-kDa protein. The 59-kDa protein bound to collagen I or collagen II that had been insolubilized on polystyrene 96-well microtiter plates, as measured by enzyme-linked immunosorbent assay. Analogously, binding to the collagens was demonstrated for the PG-S2 low Mr proteoglycan, previously shown only to inhibit collagen fibrillogenesis. The two matrix components showed similar strength of binding, i.e. Kd 35 nM for the 59-kDa protein and 16 nM for PG-S2 at 20 degrees C. The results do not reveal if the collagen interaction site of the 59-kDa protein is different from that of PG-S2. Our observations do, however, suggest that the 59-kDa protein, as well as PG-S2, have functions related to the regulation of collagen organization in tissues.

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Metadata

Authors
Hedbom, E., & Heinegård, D.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1989-04
Volume
264
Issue
12
Pages
6898-6905
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(18)83516-5
OpenAlex
W1748510480
Cited by
332
References
31
FWCI
16.49
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Interaction of a 59-kDa Connective Tissue Matrix Protein with Collagen I and Collagen II. https://app.cochat.ai/papers/10.1016/s0021-9258(18)83516-5
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Hedbom, E., & Heinegård, D. (1989). Interaction of a 59-kDa Connective Tissue Matrix Protein with Collagen I and Collagen II. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)83516-5

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.