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The Pseudomonas oleovorans alkane hydroxylase gene
Open accessAbstract
We have identified and sequenced the Pseudomonas OCT plasmid-encoded alkane hydroxylase gene (alkB) and its promoter. The transcription initiation site of the alkBAC mRNA was determined by nuclease S1 mapping. A putative interaction site with RNA-polymerase was identified based on homology of the alk promoter with other Pseudomonas promoters. The alkB gene encodes a 401-amino acid polypeptide which, despite an unusual codon composition, can be expressed at high levels in Escherichia coli and Pseudomonas. The amino-terminal sequence of the purified cytoplasmic membrane alkane hydroxylase was determined and was found to be in agreement with the nucleotide sequence. The translation product of the alkB gene contains nine hydrophobic sequences of which eight are sufficiently long to be membrane-spanning segments. The amino-terminal sequence resembles that of several bacterial integral membrane proteins and is not cleaved off following translation.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
DESATURATION AND RELATED MODIFICATIONS OF FATTY ACIDS
Shanklin, J., & Cahoon, E. B. (1998) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.49.1.611 · Cited by 916
Aarts, M. G., Keijzer, C. J., Stiekema, W. J., & Pereira, A. (1995) · The Plant Cell · doi:10.1105/tpc.7.12.2115 · Cited by 450
Diversity of Alkane Hydroxylase Systems in the Environment
Van Beilen, J. B., Li, Z., Duetz, W. A., Smits, T. H. M., & Witholt, B. (2003) · Oil & Gas Science and Technology · doi:10.2516/ogst:2003026 · Cited by 375
Self-citationLund, E. G., Kerr, T. A., Sakai, J., Li, W.-P., & Russell, D. W. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.51.34316 · Cited by 335
Structural insights into diversity and n-alkane biodegradation mechanisms of alkane hydroxylases
Ji, Y., Mao, G., Wang, Y., & Bartlam, M. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00058 · Cited by 263
Metadata
- Authors
- Kok, M., Oldenhuis, R., van der Linden, M. P. G., Raatjes, P., Kingma, J., van Lelyveld, P. H., & Witholt, B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1989-04
- Volume
- 264
- Issue
- 10
- Pages
- 5435-5441
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2129913504
- Cited by
- 195
- References
- 1
- FWCI
- 5.39
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kok, M., Oldenhuis, R., van der Linden, M. P. G., Raatjes, P., Kingma, J., van Lelyveld, P. H., & Witholt, B. (1989). The Pseudomonas oleovorans alkane hydroxylase gene. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)83564-5
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