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The nucleotide sequence of the 17S ribosomal RNA gene of Tetrahymena thermophila and the identification of point mutations resulting in resistance to the antibiotics paromomycin and hygromycin

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Spangler, E A, Blackburn, E H·1985·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(18)88976-1
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Abstract

We have determined the complete sequence of the nuclear gene encoding the small subunit (17 S) rRNA of the ciliated protozoan Tetrahymena thermophila. The gene encodes an RNA molecule which is 1753 nucleotides in length. The sequence of the Tetrahymena small subunit rRNA is homologous to those of other eukaryotes, and the predicted secondary structure for the molecule includes features which are characteristic of eukaryotic small subunit rRNAs. We have also determined the nature of two different mutations in the Tetrahymena 17 S gene which result in resistance to the aminoglycoside antibiotics paromomycin and hygromycin. In each case we have identified a single base change near the 3' end of the rRNA, within a region that is highly evolutionarily conserved in both sequence and secondary structure. Analysis of the effects of these mutations on rRNA structure, and of the impact of these drugs on translation, should help to elucidate the role of the small subunit ribosomal RNA in ribosome function.

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Metadata

Authors
Spangler, E. A., & Blackburn, E. H.
ORCID
Blackburn, E H
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1985-05
Volume
260
Issue
10
Pages
6334-6340
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(18)88976-1
OpenAlex
W1491607388
Cited by
197
References
0
Citations by year
2025: 1 · 2023: 1 · 2021: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 2
FWCI
6.66
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (GM-32565)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Protist diversity and phylogeny
Keywords
Tetrahymena thermophila, nucleotide sequence, small subunit rRNA, paromomycin resistance, hygromycin resistance, aminoglycosides, point mutations, rRNA secondary structure, ribosome function, translation (biology)
MeSH terms
Animals, Base Sequence, Chromosome Mapping, Cinnamates, Drug Resistance, Microbial, Genes, Hygromycin B, Mutation, Nucleic Acid Conformation, Paromomycin, RNA, Ribosomal, Tetrahymena

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