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A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate.
Open accessAbstract
The enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), encoded by the aroA locus, is a target site of glyphosate inhibition in bacteria. A glyphosate-resistant aroA allele has been cloned in Escherichia coli from a mutagenized strain of Salmonella typhimurium. Subcloning of this mutant aroA allele shows the gene to reside on a 1.3-kilobase segment of S. typhimurium DNA. Nucleotide sequence analysis of this mutant gene indicates a protein-coding region 427 amino acids in length. Comparison of the mutant and wild type aroA gene sequences reveals a single base pair change resulting in a Pro to Ser amino acid substitution at the 101st codon of the protein. A hybrid gene fusion between mutant and wild type aroA gene sequences was constructed. 5-Enolpyruvylshikimate-3-phosphate synthase was prepared from E. coli cells harboring this construct. The glyphosate-resistant phenotype is shown to be associated with the single amino acid substitution described above.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
The Shikimate Pathway — A Metabolic Tree with Many Branche
Bentley, R., & Haslam, E. (1990) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239009090615 · Cited by 731
The Shikimate Pathway: Early Steps in the Biosynthesis of Aromatic Compounds.
Herrmann, K. M. (1995) · The Plant Cell · doi:10.1105/tpc.7.7.907 · Cited by 592
The Biosynthetic Pathways for Shikimate and Aromatic Amino Acids in Arabidopsis thaliana
Tzin, V., & Galili, G. (2010) · The Arabidopsis Book · doi:10.1199/tab.0132 · Cited by 342
Metadata
- Authors
- Stalker, D. M., Hiatt, W. R., & Comai, L.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1985-04
- Volume
- 260
- Issue
- 8
- Pages
- 4724-4728
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1585949174
- Cited by
- 233
- References
- 1
- FWCI
- 11.65
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate. https://app.cochat.ai/papers/10.1016/s0021-9258(18)89130-x
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- Stalker, D. M., Hiatt, W. R., & Comai, L. (1985). A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)89130-x
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