The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Enzymatic Synthesis of Deoxyribonucleic Acid
Open accessAbstract
A new purification procedure for Escherichia coli DNA polymerase yields about 10 mg of homogeneous enzyme per kg of cell paste, with exonuclease III as a by-product. DNA polymerase consists of a single polypeptide chain of molecular weight 1.09 × 105. This tentative conclusion is based on (a) an unchanged molecular weight after unfolding in solutions containing guanidine hydrochloride and mercaptoethanol, (b) the presence of approximately one residue of NH2-terminal methionine, and (c) the presence of a single zone on polyacrylamide gel electrophoresis in a denaturing solvent at several pH values. The enzyme contains one disulfide bond and a single reactive sulfhydryl group, which can be modified by iodoacetic acid without change in either polymerase or its associated exonuclease activity. There is less than one phosphorus atom per molecule, thus minimizing the possibility of enzyme-associated nucleotide material in stoichiometric amounts.
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Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*
Davis, B. J. (1964) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1964.tb14213.x · Cited by 18,892
Microdetermination of Phosphorus
Chen, P. S., Toribara, T. Y., & Warner, H. (1956) · Analytical Chemistry · doi:10.1021/ac60119a033 · Cited by 7,199
DISC ELECTROPHORESIS‐I BACKGROUND AND THEORY*
Ornstein, L. (1964) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1964.tb14207.x · Cited by 4,565
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
Shapiro, A. L., Viñuela, E., & V. Maizel, J. (1967) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(67)90391-9 · Cited by 3,763
Spectroscopic Determination of Tryptophan and Tyrosine in Proteins*
Edelhoch, H. (1967) · Biochemistry · doi:10.1021/bi00859a010 · Cited by 3,436
Equilibrium Ultracentrifugation of Dilute Solutions*
Yphantis, D. A. (1964) · Biochemistry · doi:10.1021/bi00891a003 · Cited by 3,384
On the Determination of Cystine as Cysteic Acid
Moore, S. (1963) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83985-6 · Cited by 3,012
Proteins, amino acids and peptides
Oppermann, R. H. (1943) · Journal of the Franklin Institute · doi:10.1016/s0016-0032(43)91372-9 · Cited by 2,168
A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
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Cited by
Enzymatic in vitro synthesis of globin genes
Efstratiadis, A., Kafatos, F. C., Maxam, A. M., & Maniatis, T. (1976) · Cell · doi:10.1016/0092-8674(76)90027-1 · Cited by 342
Primer Requirement and Template Specificity of the DNA Polymerase of RNA Tumor Viruses
Baltimore, D., & Smoler, D. (1971) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.68.7.1507 · Cited by 229
Degradation of DNA RNA Hybrids by Ribonuclease H and DNA Polymerases of Cellular and Viral Origin
Keller, W., & Crouch, R. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.11.3360 · Cited by 222
Nucleotide sequence preservation of human mitochondrial DNA.
Monnat, R. J., & Loeb, L. A. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.9.2895 · Cited by 105
Metadata
- Authors
- Jovin, T. M., Englund, P. T., & Bertsch, L. L.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1969-06
- Volume
- 244
- Issue
- 11
- Pages
- 2996-3008
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1601321432
- Cited by
- 543
- References
- 31
- FWCI
- 68.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Jovin, T. M., Englund, P. T., & Bertsch, L. L. (1969). Enzymatic Synthesis of Deoxyribonucleic Acid. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)91722-9
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
