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Respiratory Activity of Intact, Isolated Parenchymal Cells from Rat Liver
Open accessAbstract
Abstract An enzymatic technique, with collagenase and hyaluronidase, for the preparation of isolated parenchymal cells from rat liver is described. Most of the cells (90 to 95%) appear structurally intact, but the viability of the cells varies from 75 to 95% in different preparations. The cells respire in Krebs-Ringer phosphate solution, glucose-free Hanks solution, and Medium 199, without added substrate, at rates higher than those previously described for liver cells. Furthermore, linear or near linear rates of endogenous respiration can be maintained for 2 hours. The decline of respiration which then follows is associated with certain of the cells dying off. However, after 25 hours of incubation in Medium 199, approximately 25% of the cells are still viable and respiring, and even after 3 days a few cells are still viable. Respiration is stimulated by calcium ions and by 1% albumin, but not by glucose. The results indicate that the preparative procedure for obtaining the isolated cells is far superior to those previously described.
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Citations
Cited by
Preparation of rat liver cells
Seglen, P. O. (1973) · Experimental Cell Research · doi:10.1016/0014-4827(73)90414-x · Cited by 577
A modified method for the isolation of the plasma membrane from rat liver
Ray, T. K. (1970) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(70)90159-8 · Cited by 572
Preparation of rat liver cells
Seglen, P. O. (1972) · Experimental Cell Research · doi:10.1016/0014-4827(72)90400-4 · Cited by 440
PHENOTYPIC STABILITY OF ADULT RAT HEPATOCYTES IN PRIMARY MONOLAYER CULTURE*
Bissell, D. M., & Guzelian, P. S. (1980) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1980.tb29518.x · Cited by 342
Primary Hepatocyte Isolation and Cultures: Technical Aspects, Challenges and Advancements
Kaur, I., Vasudevan, A., Rawal, P., Tripathi, D. M., Ramakrishna, S., Kaur, S., & Sarin, S. K. (2023) · Bioengineering · doi:10.3390/bioengineering10020131 · Cited by 64
Metadata
- Authors
- Howard, R. B., & Pesch, L. A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1968-06
- Volume
- 243
- Issue
- 11
- Pages
- 3105-3109
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1482331547
- Cited by
- 199
- References
- 0
- FWCI
- 25.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Howard, R. B., & Pesch, L. A. (1968). Respiratory Activity of Intact, Isolated Parenchymal Cells from Rat Liver. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)93383-1
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