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Cited by 253

The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.

Purification of the Internal Invertase of Yeast

Open access
Gascón, S, Lampen, J O·1968·Journal of Biological Chemistry

Abstract

Most of the studies on yeast invertase have been concerned only with the external enzyme (a mannan protein which is localized in the cell wall), but recently it has been reported that the invertase inside the cytoplasmic membrane is a smaller form. This paper presents a comparative study of the distribution of invertase isozymes in two stains of yeast and, after the selection of the best conditions and source, the purification of the internal or small enzyme. The major purification is obtained by chromatography on diethylaminoethyl Sephadex, although ammonium sulfate precipitation and gel filtration in Sephadex G-200 are also used to separate the large and small enzymes. The purified internal invertase is homogeneous on the basis of chromatographic criteria and its behavior in the analytical ultracentrifuge. The molecular weight of the enzyme is approximately 135,000, and the specific activity is 2,900 units per mg of protein.

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Metadata

Authors
Gascón, S., & Lampen, J. O.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1968-04
Volume
243
Issue
7
Pages
1567-1572
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1506245646
Cited by
253
References
8
FWCI
4.56
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Purification of the Internal Invertase of Yeast. https://app.cochat.ai/papers/10.1016/s0021-9258(18)93579-9
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Gascón, S., & Lampen, J. O. (1968). Purification of the Internal Invertase of Yeast. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)93579-9

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.