The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Purification of the Internal Invertase of Yeast
Open accessAbstract
Most of the studies on yeast invertase have been concerned only with the external enzyme (a mannan protein which is localized in the cell wall), but recently it has been reported that the invertase inside the cytoplasmic membrane is a smaller form. This paper presents a comparative study of the distribution of invertase isozymes in two stains of yeast and, after the selection of the best conditions and source, the purification of the internal or small enzyme. The major purification is obtained by chromatography on diethylaminoethyl Sephadex, although ammonium sulfate precipitation and gel filtration in Sephadex G-200 are also used to separate the large and small enzymes. The purified internal invertase is homogeneous on the basis of chromatographic criteria and its behavior in the analytical ultracentrifuge. The molecular weight of the enzyme is approximately 135,000, and the specific activity is 2,900 units per mg of protein.
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Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269
The gel-filtration behaviour of proteins related to their molecular weights over a wide range
Andrews, P. (1965) · Biochemical Journal · doi:10.1042/bj0960595 · Cited by 3,046
PROTEINS, AMINO ACIDS AND PEPTIDES as Ions and Dipolar Ions
COHN, E. J., EDSALL, J. T., KIRKWOOD, J. G., MUELLER, H., ONCLEY, J. L., & SCATCHARD, G. (1943) · The American Journal of the Medical Sciences · doi:10.1097/00000441-194306000-00035 · Cited by 910
Proteins, Amino Acids and Peptides as Ions and Dipolar Ions
(1944) · Physical Therapy · doi:10.1093/ptj/24.2.84a · Cited by 443
On the biological role of glycoproteins
Eylar, E. H. (1966) · Journal of Theoretical Biology · doi:10.1016/0022-5193(66)90179-2 · Cited by 442
Comparative Study of the Properties of the Purified Internal and External Invertases from Yeast
Gascón, S., Neumann, N. P., & Lampen, J. O. (1968) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)93580-5 · Cited by 280
Self-citationPurification and Properties of Yeast Invertase*
Neumann, N. P., & Lampen, J. O. (1967) · Biochemistry · doi:10.1021/bi00854a015 · Cited by 246
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Cited by
Bajaj, B. K., & Sharma, S. (2010) · Brazilian Journal of Microbiology · doi:10.1590/s1517-83822010000200030 · Cited by 20
Metadata
- Authors
- Gascón, S., & Lampen, J. O.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1968-04
- Volume
- 243
- Issue
- 7
- Pages
- 1567-1572
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1506245646
- Cited by
- 253
- References
- 8
- FWCI
- 4.56
- Type
- journal article
- Language
- en
- Open access
- hybrid
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