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Binding of Diffusible Molecules by Macromolecules: Rapid Measurement by Rate of Dialysis
Open accessAbstract
A rapid technique for the determination of the dissociation constant and the number of binding sites of an enzyme-substrate complex has been developed. The method is based on measuring the rate of dialysis of radioactive substrate from an enzyme-substrate equilibrium mixture. Accurate results can be obtained quickly, thus permitting studies with somewhat labile substances.
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Citations
Cited by
The serum transport of steroid hormones
SIITERI, P. K., MURAI, J. T., RAYMOURE, W. J., KUHN, R. W., HAMMOND, G. L., & NISKER, J. A. (1982) · Proceedings of the 1981 Laurentian Hormone Conference · doi:10.1016/b978-0-12-571138-8.50016-0 · Cited by 770
Kretsinger, R. H., & Nelson, D. J. (1976) · Coordination Chemistry Reviews · doi:10.1016/s0010-8545(00)82054-8 · Cited by 549
Concanavalin A Derivatives with Altered Biological Activities
Gunther, G. R., Wang, J. L., Yahara, I., Cunningham, B. A., & Edelman, G. M. (1973) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.70.4.1012 · Cited by 411
Mechanism of Ca2+ Activation of the NADPH Oxidase 5 (NOX5)
Bánfi, B., Tirone, F., Durussel, I., Knisz, J., Moskwa, P., Molnár, G. Z., Krause, K.-H., & Cox, J. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m310268200 · Cited by 389
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Metadata
- Authors
- Colowick, S. P., & Womack, F. C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1969-02
- Volume
- 244
- Issue
- 3
- Pages
- 774-777
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1513843435
- Cited by
- 515
- References
- 0
- FWCI
- 9.96
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Colowick, S. P., & Womack, F. C. (1969). Binding of Diffusible Molecules by Macromolecules: Rapid Measurement by Rate of Dialysis. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)94419-4
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
