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Molecular cloning and expression of human Ca(2+)-sensitive cytosolic phospholipase A2
Open accessAbstract
Phospholipases A2 (PLA2s) play a key role in inflammatory processes through production of precursors of eicosanoids and platelet-activating factor. Recently, we described the purification of a novel approximately 100-kDa cytosolic PLA2 (cPLA2) from human monoblast U937 cells that is activated by physiological (intracellular) concentrations of Ca2+ (Kramer, R. M., Roberts, E. F., Manetta, J., and Putnam, J. E. (1991) J. Biol. Chem. 266, 5268-5272). Here we report the isolation of the complementary DNA encoding human cPLA2 and confirm its identity by expression in bacteria and in hamster cells. The predicted 749-amino acid cPLA2 protein has no similarity to the well known secretory PLA2s, but contains a structural element homologous to the C2 region of protein kinase C. The molecular cloning of cPLA2 will allow further studies defining the structure, function, and regulation of this novel PLA2.
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Cited by
Nemenoff, R. A., Winitz, S., Qian, N. X., Van Putten, V., Johnson, G. L., & Heasley, L. E. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53948-x · Cited by 522
Ceramide 1-Phosphate Is a Direct Activator of Cytosolic Phospholipase A2
Pettus, B. J., Bielawska, A., Subramanian, P., Wijesinghe, D. S., Maceyka, M., Leslie, C. C., Evans, J. H., Freiberg, J., Roddy, P., Hannun, Y. A., et al. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m309262200 · Cited by 361
Mammalian non-pancreatic phospholipases A2
Kudo, I., Murakami, M., Hara, S., & Inoue, K. (1993) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(93)90003-r · Cited by 351
Arachidonic acid and free fatty acids as second messengers and the role of protein kinase C
Khan, W. A., Blobe, G. C., & Hannun, Y. A. (1995) · Cellular Signalling · doi:10.1016/0898-6568(94)00089-t · Cited by 225
Kurrasch‐Orbaugh, D. M., Parrish, J. C., Watts, V. J., & Nichols, D. E. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.01921.x · Cited by 138
Metadata
- Authors
- Sharp, J. D., White, D. L., Chiou, X. G., Goodson, T., Gamboa, G. C., McClure, D., Burgett, S., Hoskins, J., Skatrud, P. L., & Sportsman, J. R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1991-08
- Volume
- 266
- Issue
- 23
- Pages
- 14850-14853
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1563821664
- Cited by
- 448
- References
- 0
- FWCI
- 14.59
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Sharp, J. D., White, D. L., Chiou, X. G., Goodson, T., Gamboa, G. C., McClure, D., Burgett, S., Hoskins, J., Skatrud, P. L., & Sportsman, J. R. (1991). Molecular cloning and expression of human Ca(2+)-sensitive cytosolic phospholipase A2. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)98550-9
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