The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli
Open accessAbstract
A Na+/H+ antiporter coded by the nhaA (ant) gene of Escherichia coli has been overproduced and purified. The amino-terminal sequence of the protein has been determined and shown to correlate with initiation at a GUG codon, 75 bases upstream from the previously suggested AUG initiation codon. The purified protein, when reconstituted into proteoliposomes, has Na+/H+ antiport activity. It can mediate sodium uptake when a transmembrane pH gradient is applied. Downhill sodium efflux is shown to be highly dependent on pH and is accelerated by a transmembrane pH gradient. An imposed membrane potential negative inside accelerates Na+ efflux at all pH values tested. These findings suggest that the antiporter is electrogenic both at acid and alkaline pH. The activation at alkaline pH values (2000-fold increase) is consistent with the proposed role of the antiporter in regulation of internal pH at the alkaline pH range.
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Self-citationMammalian and bacterial sugar transport proteins are homologous
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Molecular aspects of bacterial pH sensing and homeostasis
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Self-citationA Bioenergetic Basis for Membrane Divergence in Archaea and Bacteria
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Vertebrate Membrane Proteins: Structure, Function, and Insights from Biophysical Approaches
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c-di-AMP, a likely master regulator of bacterial K + homeostasis machinery, activates a K + exporter
Cereija, T. B., Guerra, J. P. L., Jorge, J. M. P., & Morais-Cabral, J. H. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2020653118 · Cited by 39
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Metadata
- Authors
- Taglicht, D., Padan, E., & Schuldiner, S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1991-06
- Volume
- 266
- Issue
- 17
- Pages
- 11289-11294
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1541707791
- Cited by
- 271
- References
- 25
- FWCI
- 7.86
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Taglicht, D., Padan, E., & Schuldiner, S. (1991). Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(18)99161-1
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
