The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Expression of a synthetic gene for horseradish peroxidase C in Escherichia coli and folding and activation of the recombinant enzyme with Ca2+ and heme.
Open accessAbstract
A synthetic gene encoding horseradish peroxidase isoenzyme C (HRP C) has been synthesized and expressed in Escherichia coli. The nonglycosylated recombinant enzyme (HRP C*) was produced in inclusion bodies in an insoluble inactive form containing only traces of heme. HRP C* was solubilized and conditions under which it folded to give active enzyme were determined. Folding was shown to be critically dependent upon the concentrations of urea, Ca2+, and heme and on oxidation by oxidized glutathione. Purification of active HRP C* from the folding mixture gave a peroxidase, with about half the activity of HRP C. Glycosylation is thus not essential for correct folding and activity. The C-terminal and N-terminal extensions to HRP identified previously in cloned cDNA sequences are also not required for correct folding. However, Ca2+ appears to play a key role in folding to give the active enzyme. The overall yield of purified active enzyme was 2-3%, but this could be increased by reprocessing material that precipitated during folding.
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Cited by
Horseradish peroxidase: a modern view of a classic enzyme
Veitch, N. C. (2004) · Phytochemistry · doi:10.1016/j.phytochem.2003.10.022 · Cited by 1,379
Crystal structure of horseradish peroxidase C at 2.15 Å resolution
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Self-citationHorseradish peroxidase: a valuable tool in biotechnology
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Enzymatic Degradation of A2E, a Retinal Pigment Epithelial Lipofuscin Bisretinoid
Wu, Y., Zhou, J., Fishkin, N., Rittmann, B. E., & Sparrow, J. R. (2011) · Journal of the American Chemical Society · doi:10.1021/ja107195u · Cited by 50
Metadata
- Authors
- Smith, A. T., Santama, N., Dacey, S., Edwards, M., Bray, R. C., Thorneley, R. N., & Burke, J. F.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1990-08
- Volume
- 265
- Issue
- 22
- Pages
- 13335-13343
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1606032544
- Cited by
- 279
- References
- 33
- FWCI
- 2.03
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Smith, A. T., Santama, N., Dacey, S., Edwards, M., Bray, R. C., Thorneley, R. N., & Burke, J. F. (1990). Expression of a synthetic gene for horseradish peroxidase C in Escherichia coli and folding and activation of the recombinant enzyme with Ca2+ and heme. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)38303-6
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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