Intracellular processing of the gp160 HIV-1 envelope precursor. Endoproteolytic cleavage occurs in a cis or medial compartment of the Golgi complex
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The intracellular processing of the gp160 HIV-1 envelope precursor was characterized in acutely infected CD4+ T cells. Our data show that gp160 undergoes endoproteolytic cleavage by a nonacid dependent protease(s) in the rough endoplasmic reticulum-Golgi complex, within cis or medial cisternae, and is not transported to the cell surface. Two-dimensional electrophoretic pulse-chase analysis indicates that it takes greater than 2 h for gp160 to be transported from the rough endoplasmic reticulum to the site of action of sialyltransferases in the trans Golgi. Evidence is presented that gp160 is subject to mannose trimming in the Golgi complex, which is inhibited by 1-deoxymannojirimycin (a specific Golgi alpha-mannosidase I inhibitor). Preliminary data also suggest that gp120 is post-translationally modified by sialylated O-linked oligosaccharides.
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In HIV-1, the Env glycoproteins precursor, gp160, is cleaved in the Golgi by cellular furin or furin-like PRs into a mature surface glycoprotein gp120 and the transmembrane glycoprotein gp41.
“In case of HIV-1, the Env glycoproteins precursor, gp160, is cleaved in the Golgi by cellular furin or furin-like PRs into: (i) a mature surface glycoprotein gp120 and (ii) the transmembrane glycoprotein gp41”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Stein and Engleman 1990
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Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gpl60
Hallenberger, S., Bosch, V., Angliker, H., Shaw, E., Klenk, H.-D., & Garten, W. (1992) · Nature · doi:10.1038/360358a0 · Cited by 582 · see citing works
Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication
Jehad, M. A., Ali, L. M., Pillai, V. N., Prabhu, S. G., Mustafa, F., & Rizvi, T. A. (2026) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuaf066 · Cited by 5 · see citing works
“…HIV-1, the Env glycoproteins precursor, gp160, is cleaved in the Golgi by cellular furin or furin-like PRs into: (i) a mature surface glycoprotein gp120 and (ii) the transmembrane glycoprotein gp41 (Willey et al. 1988 , Stein and Engleman 1990 , Hallenberger et al. 1992 , Check-ley et al. 2011 ).”
- Authors
- Stein, B. S., & Engleman, E. G.
- Affiliations
- Stanford Blood Center (US)
- ORCID
- Engleman, E G
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1990-02
- Volume
- 265
- Issue
- 5
- Pages
- 2640-2649
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1529498974
- Cited by
- 189
- References
- 0
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 3 · 2013: 4 · 2012: 6
- FWCI
- 7.90
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5u01ai025922-05, 5p50hl033811-10)
- National Heart, Lung, and Blood Institute (HL33811)
- National Institute of Allergy and Infectious Diseases (AI25922)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Keywords
- HIV-1 envelope glycoprotein, gp160, endoproteolytic cleavage, Golgi apparatus, sialyltransferase, mannose trimming, 1-deoxymannojirimycin, gp120, CD4+ T cells, HIV-1 infection, glycosylation
- MeSH terms
- Cell Line, Glycoside Hydrolases, Golgi Apparatus, Humans, Kinetics, Molecular Weight, Neuraminidase, Protein Precursors, Protein Processing, Post-Translational, T-Lymphocytes, Electrophoresis, Gel, Two-Dimensional, HIV-1, Gene Products, env, HIV Envelope Protein gp160
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