The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Genomic organization of the human multidrug resistance (MDR1) gene and origin of P-glycoproteins.
Open accessAbstract
The MDR1 gene, responsible for multidrug resistance in human cells, encodes a broad specificity efflux pump (P-glycoprotein). P-glycoprotein consists of two similar halves, each half including a hydrophobic transmembrane region and a nucleotide-binding domain. On the basis of sequence homology between the N-terminal and C-terminal halves of P-glycoprotein, we have previously suggested that this gene arose by duplication of a primordial gene. We have now determined the complete intron/exon structure of the MDR1 gene by direct sequencing of cosmid clones and enzymatic amplification of genomic DNA segments. The MDR1 gene includes 28 introns, 26 of which interrupt the protein-coding sequence. Although both halves of the protein-coding sequence are composed of approximately the same number of exons, only two intron pairs, both within the nucleotide-binding domains, are located at conserved positions in the two halves of the protein. The other introns occur at different locations in the two halves of the protein and in most cases interrupt the coding sequence at different positions relative to the open reading frame. These results suggest that the P-glycoprotein arose by fusion of genes for two related but independently evolved proteins rather than by internal duplication.
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Cites
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Analysis of membrane and surface protein sequences with the hydrophobic moment plot
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The multidrug transporter, a double-edged sword.
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Self-citationA family of related ATP-binding subunits coupled to many distinct biological processes in bacteria
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Cited by
Hoffmeyer, S., Burk, O., von Richter, O., Arnold, H. P., Brockmöller, J., Johne, A., Cascorbi, I., Gerloff, T., Roots, I., Eichelbaum, M., et al. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.7.3473 · Cited by 2,365
(2002) · PubMed · Cited by 840
Synnestvedt, K., Furuta, G. T., Comerford, K. M., Louis, N., Karhausen, J., Eltzschig, H. K., Hansen, K. R., Thompson, L. F., & Colgan, S. P. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0215337 · Cited by 660
Metadata
- Authors
- Chen, C. J., Clark, D., Ueda, K., Pastan, I., Gottesman, M. M., & Roninson, I. B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1990-01
- Volume
- 265
- Issue
- 1
- Pages
- 506-514
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1587030064
- Cited by
- 269
- References
- 36
- FWCI
- 7.60
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Chen, C. J., Clark, D., Ueda, K., Pastan, I., Gottesman, M. M., & Roninson, I. B. (1990). Genomic organization of the human multidrug resistance (MDR1) gene and origin of P-glycoproteins. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)40260-3
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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