The DOI matches its Crossref record.
Two-dimensional gel analysis of soluble proteins. Characterization of guinea pig exocrine pancreatic proteins.
Open accessAbstract
A two-dimensional gel technique using slab gel isoelectric focusing in the first dimension and sodium dodecyl sulfate gradient gel electrophoresis in the second dimension has been developed for the separation of soluble proteins larger than 10,000 daltons. The technique is sensitive to 0.6 mug of protein and recovery of radiolabeled proteins averages 90%. Analysis of secretory protein from the guinea pig exocrine pancreas shows the presence of 19 distinct high molecular weight proteins. Each of these proteins has been characterized by isoelectric point, molecular weight, and proportionate mass. Thirteen of the 19 proteins have been identified by actual or potential enzymatic activity,accounting for 96% of the protein mass resolved by the two-dimensional gels.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Proteomics by Mass Spectrometry: Approaches, Advances, and Applications
Yates, J. R., Ruse, C. I., & Nakorchevsky, A. (2009) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-061008-124934 · Cited by 1,078
Proteomics: the first decade and beyond
Patterson, S. D., & Aebersold, R. H. (2003) · Nature Genetics · doi:10.1038/ng1106 · Cited by 745
Analytical techniques for cell fractions
Pearson, T., & Anderson, L. (1980) · Analytical Biochemistry · doi:10.1016/0003-2697(80)90203-1 · Cited by 622
Concepts and comparisons in multidimensional separation
Giddings, J. C. (1987) · Journal of High Resolution Chromatography · doi:10.1002/jhrc.1240100517 · Cited by 466
Pieper, R., Su, Q., Gatlin, C. L., Huang, S., Anderson, N. L., & Steiner, S. (2003) · PROTEOMICS · doi:10.1002/pmic.200390057 · Cited by 356
Metadata
- Authors
- Scheele, G. A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1975-07
- Volume
- 250
- Issue
- 14
- Pages
- 5375-5385
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1550351158
- Cited by
- 391
- References
- 0
- FWCI
- 7.36
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Two-dimensional gel analysis of soluble proteins. Characterization of guinea pig exocrine pancreatic proteins. https://app.cochat.ai/papers/10.1016/s0021-9258(19)41192-7
- The paper
- Scheele, G. A. (1975). Two-dimensional gel analysis of soluble proteins. Characterization of guinea pig exocrine pancreatic proteins. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)41192-7
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
