The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cytochrome P-450cam
Open accessAbstract
Abstract Crystalline cytochrome P-450cam, the oxygen- and sub- strate-reactive component of a selective methylene 5-exo-hydroxylase, has been prepared from camphor-induced cells of Pseudomonas putida, strain PpG786, by two similar procedures. The first involved autolysis, DEAE-cellulose chromatography, ammonium sulfate fractionation, Sephadex G-75 filtration, calcium phosphate gel chromatography, and crystallization from ammonium sulfate. Procedure II involved Bio-Gel P-150 filtration, DEAE-Sephadex and DEAE-cellulose chromatography, and ammonium sulfate fractionalization. The purified cytochrome P-450cam has a molecular weight in the range of 44,000 to 46,000, depending upon the method of determination. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the protein moiety is composed of a single polypeptide.
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Citations
Cites
The Carbon Monoxide-binding Pigment of Liver Microsomes
Omura, T., & Sato, R. (1964) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)82244-3 · Cited by 11,781
Cited by
Structure and chemistry of cytochrome p450
Denisov, I. G., Makris, T. M., Sligar, S. G., & Schlichting, I. (2005) · Chemical Reviews · doi:10.1021/cr0307143 · Cited by 2,106
Stuehr, D. J., & Ikeda-Saito, M. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)36718-3 · Cited by 347
Gunsalus, I. C., & Wagner, G. C. (1978) · Methods in Enzymology · doi:10.1016/s0076-6879(78)52019-3 · Cited by 318
Self-citationReductive dehalogenation by cytochrome P450CAM: Substrate binding and catalysis
Li, S., & Wackett, L. P. (1993) · Biochemistry · doi:10.1021/bi00087a014 · Cited by 80
The effect of cytochrome P-450cam on the NMR relaxation rate of water protons.
Philson, S. B., Debrunner, P. G., Schmidt, P. G., & Gunsalus, I. C. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)86689-9 · Cited by 49
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Metadata
- Authors
- Yu, C.-A., Gunsalus, I. C., Katagiri, M., Suhara, K., & Takemori, S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1974-01
- Volume
- 249
- Issue
- 1
- Pages
- 94-101
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1595328936
- Cited by
- 161
- References
- 1
- FWCI
- 23.70
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Yu, C.-A., Gunsalus, I. C., Katagiri, M., Suhara, K., & Takemori, S. (1974). Cytochrome P-450cam. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)43095-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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