The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Purification and characterization of leader (signal) peptidase from Escherichia coli.
Open accessAbstract
Many membrane proteins and secreted proteins are synthesized in precursor form with 15 to 30 additional NH2-terminal residues. These "leader peptides" (pre-pieces, signal peptides) are removed as these proteins cross or insert into cellular membranes. "Leader peptidase" activities which catalyze this cleavage have been detected in crude extracts and found to be dependent on membrane fractions. We now describe a 6,000-fold purification of a leader peptidase from the membranes of uninfected Escherichia coli. This leader peptidase was assayed by its ability to cleave the 23-residue leader peptide from procoat, the precursor to bacteriophage M13 coat protein. Immunoprecipitation and amino acid sequencing showed that this enzyme cleaved procoat to produce authentic coat protein. No factors other than the leader peptidase were found to be required for the conversion of procoat protein to coat protein.
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Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Cited by
Lugtenberg, B., & Van Alphen, L. (1983) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(83)90014-x · Cited by 759
Paetzel, M., Karla, A., Strynadka, N. C. J., & Dalbey, R. E. (2002) · Chemical Reviews · doi:10.1021/cr010166y · Cited by 440
β-Barrel Membrane Protein Assembly by the Bam Complex
Hagan, C. L., Silhavy, T. J., & Kahne, D. (2011) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-061408-144611 · Cited by 317
Wolfe, P. B., Wickner, W., & Goodman, J. M. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44342-0 · Cited by 302
Self-citationThe Sec translocon mediated protein transport in prokaryotes and eukaryotes.
Denks, K., Vogt, A., Sachelaru, I., Petriman, N.-A., Kudva, R., & Koch, H.-G. (2014) · Molecular Membrane Biology · doi:10.3109/09687688.2014.907455 · Cited by 170
Metadata
- Authors
- Zwizinski, C., & Wickner, W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1980-08
- Volume
- 255
- Issue
- 16
- Pages
- 7973-7977
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W175935117
- Cited by
- 235
- References
- 1
- FWCI
- 18.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Zwizinski, C., & Wickner, W. (1980). Purification and characterization of leader (signal) peptidase from Escherichia coli. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)43928-8
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
