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Amino-terminal Sequence Analysis of Proteins Purified on a Nanomole Scale by Gel Electrophoresis
Open accessAbstract
Abstract A simple, rapid, manual technique is described for determining the amino-terminal amino acid sequence of proteins on a nanomole scale. In this modification of the 5-dimethylaminonaphthalene-1-sulfonyl-Edman degradation, inorganic carriers permit convenient manipulation of small amounts of protein, and use of the detergent sodium dodecyl sulfate throughout the procedure maintains protein solubility. Nanomole quantities of pure protein for such sequence analysis are readily isolated from multicomponent systems by analytical scale polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Proteins are recovered quantitatively from the gel by elution. The method is therefore suitable for characterization of the proteins derived from multichain enzymes and viruses.
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Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
Vandekerckhove, J., & Weber, K. (1978) · Journal of Molecular Biology · doi:10.1016/0022-2836(78)90020-7 · Cited by 731
Self-citationCharacterization of the Major Envelope Protein from Escherichia coli
Rosenbusch, J. P. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42066-8 · Cited by 729
WEBER, K., & OSBORN, M. (1975) · The Proteins · doi:10.1016/b978-0-12-516301-9.50007-3 · Cited by 532
Self-citationPurification and characterization of smooth muscle myosin light chain kinase.
Adelstein, R. S., & Klee, C. B. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)68990-8 · Cited by 502
Purification and Properties of Rat Liver Microsomal Stearyl Coenzyme A Desaturase
Strittmatter, P., Spatz, L., Corcoran, D., Rogers, M. J., Setlow, B., & Redline, R. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.11.4565 · Cited by 469
Metadata
- Authors
- Weiner, A. M., Platt, T., & Weber, K.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1972-05
- Volume
- 247
- Issue
- 10
- Pages
- 3242-3251
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1561943245
- Cited by
- 647
- References
- 1
- FWCI
- 46.63
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Weiner, A. M., Platt, T., & Weber, K. (1972). Amino-terminal Sequence Analysis of Proteins Purified on a Nanomole Scale by Gel Electrophoresis. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)45237-x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
