The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Activation of the second promoter of the transforming growth factor-β1 gene by transforming growth factor-β1 and phorbol ester occurs through the same target sequences
Open accessAbstract
Two distinct regions of the transforming growth factor-beta 1 (TGF-beta 1) promoter are responsive to autoregulation and activation by phorbol ester (12-O-tetradecanoylphorbol-13-acetate): sequences located between nucleotides -454 to -323 (first promoter) and between the two transcriptional start sites. We have now characterized in detail the induction of the second promoter (sequences between nucleotides + 1 to +271) of the TGF-beta 1 gene by both TGF-beta 1 and phorbol ester. By assaying progressively deleted mutations in the second promoter, we have found two regions responsible for the induction; each contains a phorbol ester-responsive element. In vitro transcription of the second promoter-chloramphenicol acetyltransferase chimeric genes using nuclear extracts of A-549 cells showed that deletion of the putative phorbol ester-responsive elements results in a 70-80% decrease in activity. DNase I footprinting and gel mobility shift assays showed that binding to an Sp1 site and the putative TRE elements are required for maximal expression of the second promoter region of the TGF-beta 1 gene. These results suggest that AP-1, which is capable of conferring phorbol ester or TGF-beta 1 responsiveness, is the major transcription factor involved in the second promoter-derived transcription of the TGF-beta 1 gene.
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Cites
Seiki, M., Hattori, S., Hirayama, Y., & Yoshida, M. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.12.3618 · Cited by 1,525
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Poli, G. (2000) · Molecular Aspects of Medicine · doi:10.1016/s0098-2997(00)00004-2 · Cited by 631
Cytokine and cytokine receptor gene polymorphisms and their functionality
Smith, A. J. P., & Humphries, S. E. (2009) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2008.11.006 · Cited by 374
Autoinduction of transforming growth factor beta 1 is mediated by the AP-1 complex.
Kim, S. J., Angel, P., Lafyatis, R., Hattori, K., Kim, K. Y., Sporn, M. B., Karin, M., & Roberts, A. B. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.4.1492 · Cited by 317
Self-citationInagaki, Y., Truter, S., & Ramirez, F. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36699-1 · Cited by 307
Metadata
- Authors
- Kim, S. J., Denhez, F., Kim, K. Y., Holt, J. T., Sporn, M. B., & Roberts, A. B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1989-11
- Volume
- 264
- Issue
- 32
- Pages
- 19373-19378
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1570035884
- Cited by
- 183
- References
- 1
- FWCI
- 3.47
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kim, S. J., Denhez, F., Kim, K. Y., Holt, J. T., Sporn, M. B., & Roberts, A. B. (1989). Activation of the second promoter of the transforming growth factor-β1 gene by transforming growth factor-β1 and phorbol ester occurs through the same target sequences. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)47311-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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