The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Fluorescent indicators for cytosolic sodium
Open accessAbstract
Fluorescent indicators sensitive to cytosolic concentrations of free Na+ have been synthesized and characterized. They consist of a crown ether, 1,7-diaza-4,10,13-trioxacyclopentadecane, linked via its nitrogens to fluorophores bearing additional liganding centers. In the currently preferred dye, SBFI (short for sodium-binding benzofuran isophthalate), the fluorophores are benzofurans linked to isophthalate groups. Selectivities for Na+ over K+ of about 20 are observed, resulting in effective dissociation constants for Na+ of about 20 mM against a background of 120 mM K+. Increasing [Na+] increases the ratio of excitation efficiency at 330-345 nm to that at 370-390 nm with emission collected at 450-550 nm, so that ratio fluorometry and imaging can be performed with the same wavelengths as used with the well known Ca2+ indicator fura-2. If the macrocyclic ring is increased in size to a 1,10-diaza-4,7,13,16-tetraoxacyclooctadecane, the chelators become selective for K+ over Na+.
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“to sodium ions as it does to potassium ions, which makes its readout difficult to interpret.(32) Further, PBFI has a Kd of o10mM and its response saturates at approximately 50mM,”
Background
Metadata
- Authors
- Minta, A., & Tsien, R. Y.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1989-11
- Volume
- 264
- Issue
- 32
- Pages
- 19449-19457
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1595593492
- Cited by
- 658
- References
- 14
- FWCI
- 12.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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