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The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP binding protein.
Open accessAbstract
A membrane-bound protein cofactor (ARF) is required for the cholera toxin-dependent ADP-ribosylation of the stimulatory regulatory component (Gs) of adenylate cyclase. Improved methods for the purification of ARF from bovine brain are described. ARF has a high-affinity binding site for guanine nucleotides. Binding of GTP or GTP gamma S to ARF is necessary for the activity of the cofactor; GDP X ARF does not support ADP-ribosylation of Gs. Although the protein as purified contains stoichiometric amounts of GDP, GTPase activity of isolated ARF was not detected. Cholera toxin-dependent activation of adenylate cyclase thus requires two guanine nucleotide binding proteins.
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Citations
Cited by
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D'Souza-Schorey, C., & Chavrier, P. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1910 · Cited by 1,378
Helms, J. B., & Rothman, J. E. (1992) · Nature · doi:10.1038/360352a0 · Cited by 746
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Cox, A. D., & Der, C. J. (2010) · Small GTPases · doi:10.4161/sgtp.1.1.12178 · Cited by 706
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Casey, P. J., & Gilman, A. G. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)69103-3 · Cited by 608
Self-citationStamnes, M. A., & Rothman, J. E. (1993) · Cell · doi:10.1016/0092-8674(93)90277-w · Cited by 416
Metadata
- Authors
- Kahn, R. A., & Gilman, A. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1986-06
- Volume
- 261
- Issue
- 17
- Pages
- 7906-7911
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1516922966
- Cited by
- 540
- References
- 0
- FWCI
- 12.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP binding protein. https://app.cochat.ai/papers/10.1016/s0021-9258(19)57489-0
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- Kahn, R. A., & Gilman, A. G. (1986). The protein cofactor necessary for ADP-ribosylation of Gs by cholera toxin is itself a GTP binding protein. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)57489-0
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