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Purification of human erythropoietin.
Open accessAbstract
Human erythropoietin, derived from urine of patients with aplastic anemia, has been purified to apparent homogeneity. The seven-step procedure, which included ion exchange chromatography, ethanol precipitation, gel filtration, and adsorption chromatography, yielded a preparation with a potency of 70,400 units/mg of protein in 21% yield. This represents a purification factor of 930. The purified hormone has a single electrophoretic component in polyacrylamide gels at pH 9, in the presence of sodium dodecylsulfate at pH 7, and in the presence of Triton X-100 at pH 6. Two fractions of the same potency and molecular size, by sodium dodecyl sulfate gel electrophoresis, but differing slightly in mobility at pH 9, were obtained at the last step of fractionation. The nature of the difference between these two components is not yet understood.
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Cited by
Targeting Hypoxia-Inducible Factors for the Treatment of Anemia in Chronic Kidney Disease Patients
Locatelli, F., Fishbane, S., Block, G. A., & Macdougall, I. C. (2017) · American Journal of Nephrology · doi:10.1159/000455166 · Cited by 4,813
Isolation and characterization of genomic and cDNA clones of human erythropoietin
Jacobs, K., Shoemaker, C., Rudersdorf, R., Neill, S. D., Kaufman, R. J., Mufson, A., Seehra, J., Jones, S. S., Hewick, R., Fritsch, E. F., et al. (1985) · Nature · doi:10.1038/313806a0 · Cited by 1,080
Self-citationBabitt, J. L., & Lin, H. Y. (2012) · Journal of the American Society of Nephrology · doi:10.1681/asn.2011111078 · Cited by 1,041
Bartley, T. (1994) · Cell · doi:10.1016/0092-8674(94)90450-2 · Cited by 940
Krantz, S. (1991) · Blood · doi:10.1182/blood.v77.3.419.419 · Cited by 855
Metadata
- Authors
- Miyake, T., Kung, C. K., & Goldwasser, E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1977-08
- Volume
- 252
- Issue
- 15
- Pages
- 5558-5564
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1541049769
- Cited by
- 886
- References
- 0
- FWCI
- 11.46
- Type
- journal article
- Language
- en
- Open access
- hybrid
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