The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Spatial resolution of fodrin proteolysis in postischemic brain.
Open accessAbstract
One of the major obstacles in investigating in vivo proteolytic phenomena has been the inaccessibility to spatial information as to where in the tissue the reaction proceeds because the orthodox method employing electrophoretic analysis requires homogenization of samples and thus results in loss of such spatial information. To overcome this technical drawback, we have developed methodology to produce antibodies that specifically distinguish a proteolyzed form of a given protein from its intact form. Here we describe our immunohistochemical observation of calpain-catalyzed fodrin proteolysis in postischemic gerbil hippocampus, using an antibody exclusively specific to the proteolyzed 150-kDa form of fodrin alpha subunit. Our data establish a novel discovery that transient (10 min) global forebrain ischemia followed by reperfusion induces at least two distinct phases of fodrin proteolysis in hippocampus: an early phase in molecular layer and in stratum oriens of CA3 and CA1 sectors within 15 min and a late drastic and persistent phase in the entire CA1 after 4-24 h. The former may be one of the early events initiating the complex cascade leading to the delayed neuronal death, while the latter should be considered as a more direct cause for the actual degeneration in CA1.
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The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor
Kang, J., Lemaire, H.-G., Unterbeck, A., Salbaum, J. M., Masters, C. L., Grzeschik, K.-H., Multhaup, G., Beyreuther, K., & Müller-Hill, B. (1987) · Nature · doi:10.1038/325733a0 · Cited by 4,619
Vu, T.-K. H., Hung, D. T., Wheaton, V. I., & Coughlin, S. R. (1991) · Cell · doi:10.1016/0092-8674(91)90261-v · Cited by 2,970
Delayed neuronal death in the gerbil hippocampus following ischemia
Kirino, T. (1982) · Brain Research · doi:10.1016/0006-8993(82)90833-2 · Cited by 2,833
The Role of Glutamate Neurotoxicity in Hypoxic-Ischemic Neuronal Death
Choi, D. W., & Rothman, S. M. (1990) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.13.030190.001131 · Cited by 2,223
Selective vulnerability of the hippocampus in brain ischemia
Schmidt-Kastner, R., & Freund, T. F. (1991) · Neuroscience · doi:10.1016/0306-4522(91)90001-5 · Cited by 888
Postsynaptic Calcium Is Sufficient for Potentiation of Hippocampal Synaptic Transmission
Malenka, R. C., Kauer, J. A., Zucker, R. S., & Nicoll, R. A. (1988) · Science · doi:10.1126/science.2845577 · Cited by 829
The current excitement in long term potentiation
Nicoll, R. A., Kauer, J. A., & Malenka, R. C. (1988) · Neuron · doi:10.1016/0896-6273(88)90193-6 · Cited by 535
Fodrin: axonally transported polypeptides associated with the internal periphery of many cells.
Levine, J., & Willard, M. (1981) · The Journal of cell biology · doi:10.1083/jcb.90.3.631 · Cited by 475
Thilmann, R., Xie, Y., Kleihues, P., & Kiessling, M. (1986) · Acta Neuropathologica · doi:10.1007/bf00687967 · Cited by 375
Inhibition of proteolysis protects hippocampal neurons from ischemia.
Lee, K. S., Frank, S., Vanderklish, P., Arai, A., & Lynch, G. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.16.7233 · Cited by 357
Cited by
Ischemic Cell Death in Brain Neurons
Lipton, P. (1999) · Physiological Reviews · doi:10.1152/physrev.1999.79.4.1431 · Cited by 3,043
Brain ischemia and reperfusion: molecular mechanisms of neuronal injury
White, B. C., Sullivan, J. M., DeGracia, D. J., O’Neil, B. J., Neumar, R. W., Grossman, L. I., Rafols, J. A., & Krause, G. S. (2000) · Journal of the Neurological Sciences · doi:10.1016/s0022-510x(00)00386-5 · Cited by 796
Structure and physiological function of calpains
SORIMACHI, H., ISHIURA, S., & SUZUKI, K. (1997) · Biochemical Journal · doi:10.1042/bj3280721 · Cited by 707
Self-citationSaido, T. C., Iwatsubo, T., Mann, D. M. A., Shimada, H., Ihara, Y., & Kawashima, S. (1995) · Neuron · doi:10.1016/0896-6273(95)90301-1 · Cited by 587
Self-citationIn vivo imaging of axonal degeneration and regeneration in the injured spinal cord
Kerschensteiner, M., Schwab, M. E., Lichtman, J. W., & Misgeld, T. (2005) · Nature Medicine · doi:10.1038/nm1229 · Cited by 580
Metadata
- Authors
- Saido, T. C., Yokota, M., Nagao, S., Yamaura, I., Tani, E., Tsuchiya, T., Suzuki, K., & Kawashima, S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-11
- Volume
- 268
- Issue
- 33
- Pages
- 25239-25243
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1518531861
- Cited by
- 270
- References
- 32
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Saido, T. C., Yokota, M., Nagao, S., Yamaura, I., Tani, E., Tsuchiya, T., Suzuki, K., & Kawashima, S. (1993). Spatial resolution of fodrin proteolysis in postischemic brain. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)74593-1
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