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Purification and Characterization of a Protein Inhibitor of Adenosine 3',5'-Monophosphate-dependent Protein Kinases
Open accessAbstract
The partial purification and characterization of a factor from skeletal muscle which inhibits the activity of adenosine 3',5'-monophosphate-dependent protein kinases from skeletal muscle, heart, liver, adipose tissue, and brain is described. The inhibitor is stable to heating at 96° and to precipitation with 5%, w/v, of trichloracetic acid, but is assumed to be a protein since it is inactivated by proteolytic enzymes. It has a molecular weight of 26,000 by gel exclusion and an S20,w of 1.5 by sucrose density gradient centrifugation. A kinetic analysis of the effect of the inhibitor on the phosphorylation of casein by skeletal muscle protein kinase indicates that it interacts noncompetitively with respect to ATP, the protein substrate, and adenosine 3',5'-monophosphate. The inhibitor promotes a 5-fold increase in the binding constant of adenosine 3',5'-monophosphate to the protein kinase.
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Cited by
HUANG, F. L., & GLINSMANN, W. H. (1976) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1976.tb11032.x · Cited by 441
Adelstein, R. S., Conti, M. A., Hathaway, D. R., & Klee, C. B. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34295-3 · Cited by 384
Cyclic 3′ : 5′-Nucleotide Phosphodiesterase
Lin, Y. M., Liu, Y. P., & Cheung, W. Y. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42412-5 · Cited by 379
Role of calcium in the hormonal regulation of liver metabolism
Williamson, J. R., Cooper, R. H., & Hoek, J. B. (1981) · Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics · doi:10.1016/0304-4173(81)90012-4 · Cited by 317
Cyclic Nucleotide-Dependent Protein Kinases: Intracellular Receptors for cAMP and cGMP Action
Francis, S. H., & Corbin, J. D. (1999) · Critical Reviews in Clinical Laboratory Sciences · doi:10.1080/10408369991239213 · Cited by 305
Metadata
- Authors
- Walsh, D. A., Ashby, C. D., Gonzalez, C., Calkins, D., Fischer, E. H., & Krebs, E. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1971-04
- Volume
- 246
- Issue
- 7
- Pages
- 1977-1985
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2141449078
- Cited by
- 887
- References
- 0
- FWCI
- 13.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Walsh, D. A., Ashby, C. D., Gonzalez, C., Calkins, D., Fischer, E. H., & Krebs, E. G. (1971). Purification and Characterization of a Protein Inhibitor of Adenosine 3',5'-Monophosphate-dependent Protein Kinases. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)77177-4
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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