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Spacing of the -10 and -35 regions in the tac promoter. Effect on its in vivo activity.
Open accessAbstract
In the tac promoter (deBoer, H. A., Comstock, L. J., and Vasser, M. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 21-25) the spacing between the -35 and -10 consensus sequences is 16 base pairs. Between these two regions we inserted 1 or 2 base pairs to increase the distance to 17 base pairs (trc promoter) or 18 base pairs (tic promoter). The activities of the three promoters were compared in vivo by fusion to the chloramphenicol acetyltransferase or to the Escherichia coli 4.5 S RNA gene. Both measurements gave consistent results. The trc and tic promoters are on average about 90% and 65% as active as the tac promoter, respectively.
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Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
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Huang, H.-H., Camsund, D., Lindblad, P., & Heidorn, T. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq164 · Cited by 349
Khan, S. R., Gaines, J., Roop, R. M., & Farrand, S. K. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.01098-08 · Cited by 331
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Metadata
- Authors
- Brosius, J., Erfle, M., & Storella, J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1985-03
- Volume
- 260
- Issue
- 6
- Pages
- 3539-3541
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1708105888
- Cited by
- 251
- References
- 1
- FWCI
- 5.09
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Brosius, J., Erfle, M., & Storella, J. (1985). Spacing of the -10 and -35 regions in the tac promoter. Effect on its in vivo activity. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)83655-4
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