The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Determination of the mitochondrial protonmotive force in isolated hepatocytes.
Open accessAbstract
The intracellular distribution of lipophilic ions and weak acids and bases was studied in intact hepatocytes as indicators of gradients of pH and membrane potential. Mitochondrial and plasma membrane potentials of 161 and 32.8 mV (negative inside), respectively, were calculated from the intracellular accumulation of triphenylmethylphosphonium (TPMP+) and thiocyanate in the intact hepatocyte, assuming a two-compartment model. The predominantly mitochondrial localization of TPMP+ was confirmed using disruption procedures that selectively break the plasma membrane but not the mitochondrial membrane. The intracellular distribution of 5,5-dimethyloxazolidine-2,4-dione and methylamine was studied with similar techniques. A cytosolic pH of 6.95 to 7.00 and a mitochondrial delta pH of 0.97 units was observed. From these results, a total mitochondrial protonmotive force in the intact cell in excess of -200 mV was obtained.
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Cites
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HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS
Berry, M. N., & Friend, D. S. (1969) · The Journal of Cell Biology · doi:10.1083/jcb.43.3.506 · Cited by 4,424
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue
de Duve, C., Pressman, B. C., Gianetto, R., Wattiaux, R., & Appelmans, F. (1955) · Biochemical Journal · doi:10.1042/bj0600604 · Cited by 4,174
Ohkuma, S., & Poole, B. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.7.3327 · Cited by 2,174
Lysosomes in biology and pathology
(1977) · Food and Cosmetics Toxicology · doi:10.1016/s0015-6264(77)80214-9 · Cited by 1,968
CORRELATED MORPHOMETRIC AND BIOCHEMICAL STUDIES ON THE LIVER CELL
Weibel, E. R., Stäubli, W., Gnägi, H. R., & Hess, F. A. (1969) · The Journal of Cell Biology · doi:10.1083/jcb.42.1.68 · Cited by 1,141
Cytosolic phosphorylation potential.
Veech, R. L., Lawson, J. W., Cornell, N. W., & Krebs, H. A. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50401-4 · Cited by 921
NICHOLLS, D. G. (1974) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1974.tb03899.x · Cited by 717
Self-citationWaddell, W. J., & Butler, T. C. (1959) · Journal of Clinical Investigation · doi:10.1172/jci103852 · Cited by 685
Progress in surface and membrane science
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Cited by
Zielonka, J., Joseph, J., Sikora, A., Hardy, M., Ouari, O., Vasquez-Vivar, J., Cheng, G., Lopez, M., & Kalyanaraman, B. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00042 · Cited by 1,683
Mitochondria and neuronal survival
Nicholls, D. G., & Budd, S. L. (2000) · Physiological Reviews · doi:10.1152/physrev.2000.80.1.315 · Cited by 1,243
Self-citationSanderson, T. H., Reynolds, C. A., Kumar, R., Przyklenk, K., & Hüttemann, M. (2013) · Molecular Neurobiology · doi:10.1007/s12035-012-8344-z · Cited by 639
LAMBERT, A. J., & BRAND, M. D. (2004) · Biochemical Journal · doi:10.1042/bj20040485 · Cited by 470
Halestrap, A. P. (1989) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(89)80378-0 · Cited by 440
Metadata
- Authors
- Hoek, J. B., Nicholls, D. G., & Williamson, J. R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1980-03
- Volume
- 255
- Issue
- 4
- Pages
- 1458-1464
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1533884306
- Cited by
- 267
- References
- 28
- FWCI
- 8.02
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hoek, J. B., Nicholls, D. G., & Williamson, J. R. (1980). Determination of the mitochondrial protonmotive force in isolated hepatocytes. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(19)86052-0
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
