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Isolation and Chemical Characterization of a Mitogenic Lectin from Pisum sativum
Open accessAbstract
Abstract A mitogenic lectin has been isolated from garden peas (Pisum sativum) by affinity chromatography on Sephadex. The lectin has a molecular weight of 49,000, consists of two pairs of noncovalently linked polypeptide subunits and contains less than 0.5% by weight of carbohydrate. The small subunit (α) has a molecular weight of 7,000 and the large subunit (β) a molecular weight of 17,000. Examination of the tryptic digests of the subunits showed that the α subunit is not a fragment of the β subunit produced by a specific proteolytic cleavage. Pea lectin purified by affinity chromatography is a mixture of two closely related proteins which have the same subunit structure but which differ on a charge basis. The more acidic component (A) of pea lectin was present in about half the amount of the more basic component (B). The charge differences between the two components result exclusively from differences in their α subunits. Pea lectin has two binding sites for mannose (Ka = 1.4 x 103 liters per mole) and methyl α-d-glucoside (Ka = 0.8 x 103 liters per mole).
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Cited by
The Lectins: Carbohydrate-Binding Proteins of Plants and Animals
Goldstein, I. J., & Hayes, C. E. (1978) · Advances in Carbohydrate Chemistry and Biochemistry · doi:10.1016/s0065-2318(08)60220-6 · Cited by 1,621
Isolation, Physicochemical Characterization, and Carbohydrate-Binding Specificity of Lectins
GOLDSTEIN, I. J., & PORETZ, R. D. (1986) · The Lectins · doi:10.1016/b978-0-12-449945-4.50007-5 · Cited by 622
Isoelectric points and molecular weights of proteins
Righetti, P. G., & Caravaggio, T. (1976) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)98537-6 · Cited by 526
High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
The major albumin proteins from pea (Pisum sativum L). Purification and some properties
Croy, R. R. D., Hoque, M. S., Gatehouse, J. A., & Boulter, D. (1984) · Biochemical Journal · doi:10.1042/bj2180795 · Cited by 104
Metadata
- Authors
- Trowbridge, I. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1974-09
- Volume
- 249
- Issue
- 18
- Pages
- 6004-6012
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1591050105
- Cited by
- 171
- References
- 0
- FWCI
- 3.26
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Trowbridge, I. S. (1974). Isolation and Chemical Characterization of a Mitogenic Lectin from Pisum sativum. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(20)79918-7
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