The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
Selection of peptides binding to the alpha 5 beta 1 integrin from phage display library.
Open accessAbstract
The alpha 5 beta 1 integrin binds fibronectin through the integrin recognition sequence Arg-Gly-Asp (RGD). We have used a 6-amino acid peptide library expressed on filamentous phage to identify peptide ligands for alpha 5 beta 1. We found that this integrin selectively binds RGD-containing peptides from the library. Of the 32 different sequences obtained, 28 had the RGD motif, 3 contained sequences related to RGD, and only 1 had a clearly different sequence. One of the RGD-containing phage encoded a potentially cyclic insert CRGDCL. The cyclic peptide GAC*RGDC*LGA (where * denotes cysteines forming a disulfide bond) was 10-fold more efficient than any of the linear RGD-containing hexapeptides in inhibiting the binding of RGD-expressing phage to alpha 5 beta 1 or the attachment of alpha 5 beta 1-expressing cells to fibronectin. This peptide also inhibited cell attachment mediated by the alpha v beta 1, alpha v beta 3, and alpha v beta 5 integrins with about 10-fold higher efficiency than linear GRGDSP. One peptide containing an RGD-related sequence, NGRAHA, was also found to inhibit phage attachment and cell adhesion, especially adhesion mediated by the alpha v beta 5 integrin. These results indicate that novel and high affinity ligands for integrins can be isolated from a random peptide library.
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Integrins: Versatility, modulation, and signaling in cell adhesion
Hynes, R. O. (1992) · Cell · doi:10.1016/0092-8674(92)90115-s · Cited by 9,857
New Perspectives in Cell Adhesion: RGD and Integrins
Ruoslahti, E. I., & Pierschbacher, M. D. (1987) · Science · doi:10.1126/science.2821619 · Cited by 4,875
Self-citationPierschbacher, M. D., & Ruoslahti, E. I. (1984) · Nature · doi:10.1038/309030a0 · Cited by 3,690
Self-citationSearching for Peptide Ligands with an Epitope Library
Scott, J. K., & Smith, G. P. (1990) · Science · doi:10.1126/science.1696028 · Cited by 2,087
Integrins and other cell adhesion molecules
Albelda, S. Μ., & Buck, C. A. (1990) · The FASEB Journal · doi:10.1096/fasebj.4.11.2199285 · Cited by 1,975
Ruoslahti, E. I. (1991) · Journal of Clinical Investigation · doi:10.1172/jci114957 · Cited by 1,391
Self-citationPytela, R., Pierschbacher, M. D., & Ruoslahti, E. I. (1985) · Cell · doi:10.1016/0092-8674(85)90322-8 · Cited by 1,101
Self-citationPeptides on phage: a vast library of peptides for identifying ligands.
Cwirla, S. E., Peters, E. A., Barrett, R. W., & Dower, W. J. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.16.6378 · Cited by 930
Random Peptide Libraries: a Source of Specific Protein Binding Molecules
Devlin, J. J., Panganiban, L. C., & Devlin, P. E. (1990) · Science · doi:10.1126/science.2143033 · Cited by 923
Antibody-selectable filamentous fd phage vectors: affinity purification of target genes
Parmley, S. F., & Smith, G. P. (1988) · Gene · doi:10.1016/0378-1119(88)90495-7 · Cited by 871
Research works printed but not linked: 2
- Saiki, 1989, Cancer Res., 49, 3815
- Schreiner, 1991, Cancer Res., 51, 1738
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Lee, L. A., Balizan, E., Lin, Y., & Wang, Q. (2012) · ACS symposium series · doi:10.1021/bk-2012-1119.ch002 · Cited by 2
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Cavalcanti‐Adam, E. A., & Missirlis, D. (2012) · doi:10.1002/9783527652273.ch9 · Cited by 2
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Niculescu‐Duvaz, D., Springer, C. J., & Marais, R. (2010) · Pigment Cell & Melanoma Research · doi:10.1111/j.1755-148x.2010.00675.x · Cited by 2
Overview: Biologicals & Immunologicals; Biomolecule libraries, arrays and molecular diversity
Harris, C. J. (1995) · Expert Opinion on Therapeutic Patents · doi:10.1517/13543776.5.5.469 · Cited by 2
Integrins: cell adhesion molecules in cancer
Fields, G. (1998) · Expert Opinion on Therapeutic Patents · doi:10.1517/13543776.8.6.633 · Cited by 2
Distinct roles for astrocyte αvβ5 and αvβ8 integrins in adhesion and migration
Milner, R., Huang, X., Wu, J., Nishimura, S. L., Pytela, R., Sheppard, D., & ffrench‐Constant, C. (1999) · Journal of Cell Science · doi:10.1242/jcs.112.23.4271 · Cited by 1
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Ozawa, M. G., Staquicini, F. I., Sidman, R. L., Pasqualini, R., & Arap, W. (2009) · Humana Press eBooks · doi:10.1007/978-1-60327-553-8_32 · Cited by 1
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Hung, J. S.-C., Reynolds, F., & Kelly, K. A. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-816386-3.00062-4 · Cited by 1
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Lui, B. H., & Cochran, J. R. (2011) · Comprehensive Biomaterials · doi:10.1016/b978-0-08-055294-1.00258-0
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Srinivasan, S. (2014) · ERA: Education and Research Archive (University of Alberta) · doi:10.7939/r3nm33
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Lam, M. (2011) · ERA: Education and Research Archive (University of Alberta) · doi:10.7939/r3fh1f
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Ravi, S. (2010) · SMARTech Repository (Georgia Institute of Technology)
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Prakash, V. S., & Ramakrishnan, V. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-99917-5.00003-2
Data from Species D Adenoviruses as Oncolytics against B-cell Cancers
Chen, C. Y., Senac, J. S., Weaver, E. A., May, S. M., Jelinek, D. F., Greipp, P. R., Witzig, T. E., & Barry, M. A. (2023) · doi:10.1158/1078-0432.c.6519689.v1
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Chen, C. Y., Senac, J. S., Weaver, E. A., May, S. M., Jelinek, D. F., Greipp, P. R., Witzig, T. E., & Barry, M. A. (2023) · doi:10.1158/1078-0432.c.6519689
Leblanc, E., Moal, E. L., Liu, Y., Théroux, L., Bonin, M., Schüler, S. C., Boudreault, P., & Bentzinger, F. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4621559
Benwell, C. J., Johnson, R., Taylor, J., Lambert, J., & Robinson, S. D. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.11.28.568967
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Metadata
- Authors
- Koivunen, E., Gay, D. A., & Ruoslahti, E.
- Affiliations
- Sanford Burnham Prebys Medical Discovery Institute (US)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1993-09
- Volume
- 268
- Issue
- 27
- Pages
- 20205-20210
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1506729263
- Cited by
- 344
- References
- 62
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 4 · 2023: 8 · 2022: 3 · 2021: 8 · 2020: 5 · 2019: 2 · 2018: 6 · 2017: 8 · 2016: 15 · 2015: 8 · 2014: 9 · 2013: 11 · 2012: 14
- FWCI
- 7.09
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p01ca028896-15, 5r35ca042507-07)
- National Cancer Institute (CA30199, CA 28896, CA-42507, P30CA030199)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Keywords
- alpha5beta1 integrin, RGD peptide, fibronectin, integrin ligands, cyclic RGD peptide, phage display, cell adhesion, peptide library screening
- MeSH terms
- Amino Acid Sequence, Animals, Bacteriophages, Base Sequence, Binding, Competitive, Fibronectins, Cricetinae, Kinetics, Molecular Sequence Data, Oligodeoxyribonucleotides, Oligopeptides, Transfection, Gene Library, Integrins, Polymerase Chain Reaction, Databases, Factual, CHO Cells, Receptors, Fibronectin
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