The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Isolation and sequence analysis of a barley alpha-amylase cDNA clone.
Open accessAbstract
We have isolated a cDNA clone derived from poly(A+) RNA from barley aleurone cells stimulated with gibberellic acid. This cDNA clone contains one open reading frame coding for 438 amino acids. The cloned DNA hybridizes to a poly(A+) RNA species 1550 bases in size, the same size as the most abundant poly(A+) RNA molecules in stimulated cells. RNA complementary to this clone can be translated to make immunoprecipitable alpha-amylase in the wheat germ system and increases about 5-fold in quantity after gibberellic acid stimulation of aleurone cells. In contrast, hybridization experiments using a total cDNA probe demonstrate that the most abundant mRNA population, identical in size with our cloned sequence and presumably that for alpha-amylase, increases at least 17-fold after gibberellic acid stimulation. We therefore infer that there must be at least two populations of alpha-amylase mRNA molecules derived from separate structural genes differently influenced by gibberellic acid in aleurone cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Berger, S. L., & Birkenmeier, C. S. (1979) · Biochemistry · doi:10.1021/bi00590a018 · Cited by 443
Cited by
Comparison of amino acid sequences of eleven different ?-amylases
Nakajima, R., Imanaka, T., & Aiba, S. (1986) · Applied Microbiology and Biotechnology · doi:10.1007/bf00257032 · Cited by 291
Jobling, S. A., & Gehrke, L. (1987) · Nature · doi:10.1038/325622a0 · Cited by 278
Metadata
- Authors
- Rogers, J. C., & Milliman, C.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1983-07
- Volume
- 258
- Issue
- 13
- Pages
- 8169-8174
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1546045554
- Cited by
- 202
- References
- 2
- FWCI
- 19.75
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Isolation and sequence analysis of a barley alpha-amylase cDNA clone. https://app.cochat.ai/papers/10.1016/s0021-9258(20)82044-4
- The paper
- Rogers, J. C., & Milliman, C. (1983). Isolation and sequence analysis of a barley alpha-amylase cDNA clone. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(20)82044-4
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
