Determination of meropenem in serum by high-performance liquid chromatography with column switching
Abstract
A rapid, accurate and sensitive liquid chromatographic assay with on-line solid-phase extraction for determination of meropenem in serum is described. Sample was directly injected onto the extraction column for sample clean-up and extraction. Thereafter, using an on-line column-switching system the drug was quantitatively transferred and separated on a C18 analytical column. Ultraviolet absorption at 298 nm was used for detection. The assay was linear from 1 to 100 micrograms/ml. Recovery was 98.5%. Based on a 20-microliters sample volume (serum- water, 1:1, v/v), detection limit was 0.1 microgram/ml. An application of the method to study the pharmacokinetics of meropenem is given.
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Several HPLC assays with ultraviolet detection for the determination of these drugs, alone or in combination, have been reported.
“Several HPLC assays with ultraviolet (UV) detection for the determination of these drugs, alone or in combination, have been reported [9-17].”
These reported HPLC assays have extensive chromatographic run times.
“These assays have some disadvantages in terms of extensive chromatographic run times [9,11-13,15,16] a large consumption of non environmentally friendly solvents [11] or a large amount of sample [11,15,16].”
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Ferrone, V., Cotellese, R., Carlucci, M., Di Marco, L., & Carlucci, G. (2018) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2017.12.039 · Cited by 43 · see citing works
- Authors
- Bompadre, S., Ferrante, L., De Martinis, M., & Leone, L.
- Corresponding author
- Leone, L
- ORCID
- Ferrante, L
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 1998-07
- Volume
- 812
- Issue
- 1-2
- Pages
- 249-253
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W2071933884
- Cited by
- 28
- References
- 0
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2014: 2
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography A (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Health Sciences › Medicine › Pharmacology › Antibiotics Pharmacokinetics and Efficacy
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Keywords
- meropenem, serum drug quantification, column switching, on-line solid-phase extraction, pharmacokinetics, carbapenems, UV detection, sample cleanup
- MeSH terms
- Meropenem, Chromatography, High Pressure Liquid, Humans, Indicators and Reagents, Solvents, Spectrophotometry, Ultraviolet, Thienamycins
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