The DOI matches its Crossref record; 71 of the 73 references it cites were found in a registry or an index.
Localization of adipocyte long-chain fatty acyl-CoA synthetase at the plasma membrane
Open accessAbstract
Long-chain fatty acyl-CoA synthetase (FACS) catalyzes esterification of long-chain fatty acids (LCFAs) with coenzyme A (CoA), the first step in fatty acid metabolism. FACS has been shown to play a role in LCFA import into bacteria and implicated to function in mammalian cell LCFA import. In the present study, we demonstrate that FACS overexpression in fibroblasts increases LCFA uptake, and overexpression of both FACS and the fatty acid transport protein (FATP) have synergistic effects on LCFA uptake. To explore how FACS contributes to LCFA import, we examined the subcellular location of this enzyme in 3T3-L1 adipocytes which natively express this protein and which efficiently take up LCFAs. We demonstrate for the first time that FACS is an integral membrane protein. Subcellular fractionation of adipocytes by differential density centrifugation reveals immunoreactive and enzymatically active FACS in several membrane fractions, including the plasma membrane. Immunofluorescence studies on adipocyte plasma membrane lawns confirm that FACS resides at the plasma membrane of adipocytes, where it co-distributes with FATP. Taken together, our data support a model in which imported LCFAs are immediately esterified at the plasma membrane upon uptake, and in which FATP and FACS function coordinately to facilitate LCFA movement across the plasma membrane of mammalian cells.
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“There is abundant evidence which indicates that CD36 plays an important role in LCFA uptake into skeletal muscle in rodents and human and its concentration and activity are increased following exercise [89–96].”
From Pathogenesis of insulin resistance in skeletal muscle (2010) · cites this paper as [89]
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Self-citationRole of long-chain fatty acyl-CoA esters in the regulation of metabolism and in cell signalling
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A novel mouse model of lipotoxic cardiomyopathy
Chiu, H.-C., Kovács, A., Ford, D. A., Hsu, F., Garcia, R. A., Herrero, P., Saffitz, J. E., & Schaffer, J. E. (2001) · Journal of Clinical Investigation · doi:10.1172/jci10947 · Cited by 762
Self-citationGlatz, J. F. C., Luiken, J. J. F. P., & Bonen, A. (2010) · Physiological Reviews · doi:10.1152/physrev.00003.2009 · Cited by 776
Pathogenesis of insulin resistance in skeletal muscle
Abdul-Ghani, M. A., & DeFronzo, R. A. (2010) · Journal of Biomedicine and Biotechnology · doi:10.1155/2010/476279 · Cited by 670
Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance
Kiens, B. (2006) · Physiological Reviews · doi:10.1152/physrev.00023.2004 · Cited by 478
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Metadata
- Authors
- Gargiulo, C. E., Stuhlsatz-Krouper, S. M., & Schaffer, J. E.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Schaffer, Jean E.
- ORCID
- Schaffer, Jean E.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1999-05
- Volume
- 40
- Issue
- 5
- Pages
- 881-892
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2148427109
- Cited by
- 156
- References
- 73
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 2 · 2019: 5 · 2018: 2 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 5 · 2013: 2 · 2012: 5
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK054268, DK54268)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Keywords
- fatty acid esterification, long-chain fatty acid uptake, fatty acid transporters, plasma membrane localization, adipocytes, membrane proteins, fatty acid metabolism, 3T3-L1 adipocytes, fatty acid import
- MeSH terms
- Long-Chain-Fatty-Acid-CoA Ligase, Animals, Biological Transport, Active, Carrier Proteins, Cell Membrane, Coenzyme A Ligases, Fatty Acids, Membrane Proteins, Microscopy, Fluorescence, Models, Biological, Repressor Proteins, 3T3 Cells, Adipocytes, Membrane Transport Proteins, Saccharomyces cerevisiae Proteins, Fatty Acid Transport Proteins, Mice
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