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A microfluorometric method for the determination of free fatty acids in plasma
Open accessAbstract
A rapid and precise microfluorometric method for the determination of free fatty acid concentrations in 2-5 microliters of plasma is described. The assay is performed directly on plasma, eliminating the need for extraction with organic solvents, and is based on the quantitation of AMP generated from the formation of acyl coenzyme A in the presence of ATP and acyl CoA synthetase. Because of the sensitivity of this assay, reagent quantities, and thus costs, are significantly reduced compared with previously described enzymatic spectrophotometric methods. In addition, the inclusion of fatty acid-free human serum albumin in the standards corrects the previously reported underestimate of plasma free fatty acids with enzymic methods.
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Cited by
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Seppala-Lindroos, A. (2002) · Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.87.7.3023 · Cited by 168
Mechanism by which metformin reduces glucose production in type 2 diabetes.
Hundal, R. S., Krssak, M., Dufour, S., Laurent, D., Lebon, V, Chandramouli, V, Inzucchi, S. E., Schumann, W. C., Petersen, K. F., Landau, B. R., et al. (2000) · Diabetes · doi:10.2337/diabetes.49.12.2063 · Cited by 1,052
Petersen, K. F., Dufour, S., Befroy, D., Lehrke, M., Hendler, R. E., & Shulman, G. I. (2005) · Diabetes · doi:10.2337/diabetes.54.3.603 · Cited by 911
Determinants of the impaired secretion of glucagon-like peptide-1 in type 2 diabetic patients
Toft-Nielsen, M.-B., Damholt, M. B., Madsbad, S., Hilsted, L. M., Hughes, T. E., Michelsen, B. K., & Holst, J. J. (2001) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.86.8.7750 · Cited by 869
Metadata
- Authors
- Miles, J., Glasscock, R., Aikens, J., Gerich, J., & Haymond, M.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1983-01
- Volume
- 24
- Issue
- 1
- Pages
- 96-99
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2129686285
- Cited by
- 329
- References
- 0
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: A microfluorometric method for the determination of free fatty acids in plasma. https://app.cochat.ai/papers/10.1016/s0022-2275(20)38029-9
- The paper
- Miles, J., Glasscock, R., Aikens, J., Gerich, J., & Haymond, M. (1983). A microfluorometric method for the determination of free fatty acids in plasma. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)38029-9
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
