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Quantitative analysis of phospholipids and phospholipid fatty acids from silica gel thin-layer chromatograms
Open accessAbstract
An improved procedure for the quantitative assay of phospholipids separated by TLC is described, in which a specially washed Silica Gel H and a newly designed chromatography unit are employed.Analysis of phospholipid phosphorus and phospholipid fatty acids was accomplished in the presence of silica gel scraped directly from the chromatoplates. Recovery of phosphorus ranged from 96 to 100%.The washing of the Silica Gel H with chloroform–methanol–formic acid resulted in extremely low phosphorus blanks on the silica gel, and also removed impurities which were found to cause various peaks on GLC.
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Citations
Cited by
Preparation and Use of Liposomes as Models of Biological Membranes
Bangham, A. D., Hill, M. W., & Miller, N. G. A. (1974) · Methods in Membrane Biology · doi:10.1007/978-1-4615-7422-4_1 · Cited by 585
[54] Thin-layer chromatography of lipids
(1969) · Methods in Enzymology · doi:10.1016/s0076-6879(69)14056-2 · Cited by 573
Quantitative densitometric thin-layer chromatography of lipids using copper acetate reagent
Fewster, M. E., Burns, B. J., & Mead, J. F. (1969) · Journal of Chromatography A · doi:10.1016/s0021-9673(00)99173-8 · Cited by 403
Role of plasma lecithin:cholesterol acyltransferase in the metabolism of high density lipoproteins
Glomset, J. A., Janssen, E. T., Kennedy, R., & Dobbins, J. (1966) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)39245-2 · Cited by 279
Dougherty, R. M., Galli, C., Ferro-Luzzi, A., & Iacono, J. M. (1987) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/45.2.443 · Cited by 249
Metadata
- Authors
- Parker, F., & Peterson, N. F.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1965-10
- Volume
- 6
- Issue
- 4
- Pages
- 455-460
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2189419402
- Cited by
- 368
- References
- 0
- FWCI
- 20.56
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Parker, F., & Peterson, N. F. (1965). Quantitative analysis of phospholipids and phospholipid fatty acids from silica gel thin-layer chromatograms. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)39605-x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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