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Colorimetric determination of free fatty acids in biological fluids
Open accessAbstract
Following the introduction by Duncombe of a colorimetric micromethod for the determination of free fatty acids (FFA) dissolved in chloroform, we studied the optimum conditions for chloroform extraction of FFA from blood or other biological fluids. A degree and rate of extraction similar to those with Dole's extraction mixture were obtained.Phospholipids are not extracted by the procedure described. As little as 0.2 ml of whole blood, instead of serum or plasma, can serve as a FFA sample in the subsequent colorimetric determination, which has been modified to render it simpler and more sensitive.
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Citations
Cited by
Laakso, M., Edelman, S. V., Brechtel, G., & Baron, A. D. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114644 · Cited by 930
Vaag, A., Henriksen, J. E., & Beck-Nielsen, H. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115656 · Cited by 299
Adverse metabolic effect of omega-3 fatty acids in non-insulin-dependent diabetes mellitus
GLAUBER, H., WALLACE, P., GRIVER, K., & BRECHTEL, G. (1988) · Annals of Internal Medicine · doi:10.7326/0003-4819-108-5-663 · Cited by 285
The fuel of respiration of rat kidney cortex
Weidemann, M. J., & Krebs, H. A. (1969) · Biochemical Journal · doi:10.1042/bj1120149 · Cited by 272
Mandarino, L. J., Wright, K. S., Verity, L. S., Nichols, J., Bell, J. M., Kolterman, O. G., & Beck-Nielsen, H. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113118 · Cited by 194
Metadata
- Authors
- Itaya, K., & Ui, M.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1965-01
- Volume
- 6
- Issue
- 1
- Pages
- 16-20
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2289466277
- Cited by
- 714
- References
- 0
- FWCI
- 6.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
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