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Cited by 261Lipid metabolism and biosynthesisLipid metabolism and disordersPeroxisome Proliferator-Activated Receptors

The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.

Hormonal regulation of hormone-sensitive lipase activity and mRNA levels in isolated rat adipocytes.

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Slavin, B G, Ong, J M, Kern, P A·1994·Journal of Lipid Research
DOI 10.1016/s0022-2275(20)41151-4
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Abstract

Hormone-sensitive lipase (HSL) mediates the lipolysis of triacylglycerol from mammalian adipocytes, resulting in the release of non-esterified fatty acids and glycerol. Although numerous studies have examined the hormonal regulation of HSL, the measurement of HSL mRNA levels in response to hormonal regulators has not been studied. This study was designed to determine the effects of epinephrine, growth hormone, glucagon, and dexamethasone on HSL expression by measuring HSL mRNA levels and glycerol release in primary cultures of rat adipocytes. Exposure of adipocytes to epinephrine at 10(-7) M and 10(-5) M for 4 h resulted in an increase in medium glycerol (209 +/- 46%, and 284 +/- 58% of control, P < 0.001, respectively). However, no change in HSL mRNA levels occurred due to the epinephrine treatment. Similarly, the peptides glucagon (10(-7) M and 10(-5) M for 4 h) and growth hormone (100 ng/ml for 24 h) resulted in increased medium glycerol and had no effect on HSL mRNA levels in adipocytes. Dexamethasone was added to adipocyte cultures for 4 and 24 h, and resulted in a dose-dependent increase of medium glycerol (102 +/- 8%, 138 +/- 8% (P < 0.001), and 168 +/- 24% (P < 0.001) for 10(-8) M, 10(-7) M, and 10(-6) M, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)

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Crossref · 10.1016/s0022-2275(20)41151-4

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Metadata

Authors
Slavin, B. G., Ong, J. M., & Kern, P. A.
Affiliations
University of Southern California (US)
ORCID
Kern, P A
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1994-09
Volume
35
Issue
9
Pages
1535-1541
ISSN
0022-2275 (ISSN-L)
DOI
10.1016/s0022-2275(20)41151-4
OpenAlex
W2185515430
Cited by
261
References
44
Citations by year
2026: 2 · 2025: 6 · 2024: 4 · 2023: 15 · 2022: 10 · 2021: 9 · 2020: 6 · 2019: 6 · 2018: 3 · 2017: 12 · 2016: 12 · 2015: 13 · 2014: 17 · 2013: 12 · 2012: 8
FWCI
2.56
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Lipid Research (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Institute of Diabetes and Digestive and Kidney Diseases (DK 39176)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords
hormone-sensitive lipase, adipocytes, lipolysis, epinephrine, glucagon, growth hormone, dexamethasone, glycerol release, hormonal regulation
MeSH terms
Adipose Tissue, Animals, Cells, Cultured, Sterol Esterase, Dexamethasone, Epinephrine, Glucagon, Glycerol, Hormones, Male, RNA Processing, Post-Transcriptional, RNA, Messenger, Growth Hormone, Gene Expression Regulation, Enzymologic, Rats

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