The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Improved quantitation of plasma lipids by direct gas-liquid chromatography
Abstract
A preliminary digestion of total plasma lipid extracts with phospholipase C, which converts the lysolecithins, lecithins, and sphingomyelins into monoglycerides, diglycerides, and ceramides, respectively, has been shown to facilitate subsequent determination of the plasma lipids by gas-liquid chromatography. A further improvement in the chromatographic elution pattern results from acetylation or trimethylsilylation of the liberated alcohol moieties prior to injection into the chromatograph. If tridecanoin is used as internal standard, quantitative estimates can be rapidly obtained for plasma lysolecithins, free cholesterol, lecithins, sphingomyelins, cholesteryl esters, and triglycerides, as well as for free fatty acids. Other plasma lipids do not occur in sufficiently high concentrations to interfere with the analysis. The determination requires 0.1-0.5 ml of plasma and about 6 hr of processing, but many samples can be processed at a time.
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“This number is about twice that of previous estimates of plasma SM subspecies”
From Lipidomics reveals a remarkable diversity of lipids in human plasma (2010) · doi:10.1194/jlr.m009449 · cites this paper as [20]
Citations
Cites
Lipid Chromatographic Analysis
(1994) · Analytica Chimica Acta · doi:10.1016/0003-2670(94)80326-9 · Cited by 657
A new method for the preparation of diazomethane
de Boer, T. J., & Backer, H. J. (1954) · Recueil des Travaux Chimiques des Pays-Bas · doi:10.1002/recl.19540730308 · Cited by 315
Individual molecular species of different phospholipid classes. Part II. A method of analysis
Renkonen, O. (1965) · Journal of the American Oil Chemists' Society · doi:10.1007/bf02540133 · Cited by 260
BIOCHEMISTRY OF THE SPHINGOLIPIDES
Carter, H. E., Glick, F. J., Norris, W. P., & Phillips, G. E. (1947) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34955-4 · Cited by 107
Gas‐liquid chromatographic analysis of monoglycerides as their trimethylsilyl ether derivatives
Wood, R., Raju, P. K., & Reiser, R. (1965) · Journal of the American Oil Chemists Society · doi:10.1007/bf02541121 · Cited by 97
Zamecnik, P. C., Brewster, L. E., & Lipmann, F. A. (1947) · The Journal of Experimental Medicine · doi:10.1084/jem.85.4.381 · Cited by 91
Gas‐liquid chromatographic analysis of long chain isomeric glyceryl monoethers
Wood, R., & Snyder, F. F. C. (1966) · Lipids · doi:10.1007/bf02668125 · Cited by 68
Beveridge, J. M. R., Jagannathan, S. N., & Connell, W. F. (1964) · Canadian Journal of Biochemistry · doi:10.1139/o64-110 · Cited by 65
Williams, J. H., Kuchmak, M., & Witter, R. F. (1966) · Lipids · doi:10.1007/bf02532997 · Cited by 53
Some structural investigations on phospholipids from membranes
Deenen, L. L. M. V. (1966) · Journal of the American Oil Chemists Society · doi:10.1007/bf02609677 · Cited by 19
Research works printed but not linked: 10
- Kuksis, 1967, J. LipidRes., 8, 352
- Phillips, 1967, J. LipidRes., 8, 676
- Kuksis, 1969, J. LipidRes., 10, 25
- Carroll, 1961, J. LipidRes., 2, 135
- Kuksis, 1967, 1, 239
- Tallent, 1968, J. LipidRes., 9, 146
- Marai, 1969, J. LipidRes., 10, 141
- Samuelsson, 1969, J. LipidRes., 10, 47
- Watts, 1969, J. LipidRes., 10, 33
- Wood, 1969, J. LipidRes., 10, 128
Cited by
Lipidomics reveals a remarkable diversity of lipids in human plasma
Quehenberger, O., Armando, A. M., Brown, A. H., Milne, S. B., Myers, D. S., Merrill, A. H., Bandyopadhyay, S., Jones, K. N., Kelly, S., Shaner, R. L., et al. (2010) · Journal of Lipid Research · doi:10.1194/jlr.m009449 · Cited by 1,341
Metadata
- Authors
- Kuksis, A., Stachnyk, O., & Holub, B. J.
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1969-11
- Volume
- 10
- Issue
- 6
- Pages
- 660-667
- ISSN
- 0022-2275
- Cited by
- 50
- References
- 12
- Language
- en
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- creativecommons.org/licenses/by/4.0/ (vor, 18620 d)
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- Kuksis, A., Stachnyk, O., & Holub, B. J. (1969). Improved quantitation of plasma lipids by direct gas-liquid chromatography. Journal of Lipid Research. https://doi.org/10.1016/s0022-2275(20)43027-5
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.