Field and laboratory comparative evaluation of ten rapid malaria diagnostic tests
Abstract
The paper reports on a comparative evaluation of 10 rapid malaria tests available in South Africa in 1998: AccuCheck (AC, developmental), Cape Biotech (CB), ICT Malaria Pf (ICT1) and Pf/Pv (ICT2), Kat Medical (KAT), MakroMal (MM), OptiMAL (OP), ParaSight-F (PS), Quorum (Q), Determine-Malaria (DM). In a laboratory study, designed to test absolute detection limits, Plasmodium falciparum-infected blood was diluted with uninfected blood to known parasite concentrations ranging from 500 to 0.1 parasites per microlitre (P/microL). The 50% detection limits were: ICT1, 3.28; ICT2, 4.86; KAT, 6.36; MM, 9.37; CB, 11.42; DM, 12.40; Q, 16.98; PS, 20; AC, 31.15 and OP, 91.16 P/microL. A field study was carried out to test post-treatment specificity. Blood samples from malaria patients were tested with all products (except AC and DM) on the day of treatment and 3 and 7 days thereafter, against a gold standard of microscopy and polymerase chain reaction (PCR). OP and PS produced fewer false-positive results on day 7 (18 and 19%, respectively) than the other rapid tests (38-56%). However, microscopy, PCR, OP and PS disagreed largely as to which individuals remained positive. The tests were further compared with regard to general specificity, particularly cross-reactivity with rheumatoid factor, speed, simplicity, their ability to detect other species, storage requirements and general presentation.
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Antigenemia may persist weeks beyond the actual infection, leading to the false diagnosis of malaria parasitemia.
“In addition, antigenemia may persist weeks beyond the actual infection, leading to the false diagnosis of malaria parasitemia (6, 22).”
From Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp (2005) · cites this paper as [6]
The persistence of Plasmodium DNA is less than the persistent antigenemia reported with immunochromatographic assays.
“However, this persistence of Plasmodium DNA is still less than the persistent antigenemia reported with immunochromatographic assays (6, 22).”
From Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp (2005) · cites this paper as [6]
Cites
Persistence of Plasmodium falciparum HRP-2 in successfully treated acute falciparum malaria
Mayxay, M., Pukrittayakamee, S., Chotivanich, K., Looareesuwan, S., & White, N. J. (2001) · Transactions of the Royal Society of Tropical Medicine and Hygiene · doi:10.1016/s0035-9203(01)90156-7 · Cited by 175 · see citing works
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Real-Time PCR for Detection and Identification of <i>Plasmodium</i> spp
Mangold, K. A., Manson, R. U., Koay, E. S. C., Stephens, L., Regner, M., Thomson, R. B., Peterson, L. R., & Kaul, K. L. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.5.2435-2440.2005 · Cited by 278 · see citing works
Maltha, J., Gillet, P., Bottieau, E., Cnops, L., van Esbroeck, M., & Jacobs, J. (2010) · Malaria Journal · doi:10.1186/1475-2875-9-171 · Cited by 87 · see citing works
Singh, N., Shukla, M. M., Shukla, M. K., Mehra, R. K., Sharma, S., Bharti, P. K., Singh, M. P., Singh, A., & Gunasekar, A. (2010) · Malaria Journal · doi:10.1186/1475-2875-9-191 · Cited by 76 · see citing works
- Authors
- Craig, M. H., Bredenkamp, B. L., Williams, C. H. V., Rossouw, E. J., Kelly, V. J., Kleinschmidt, I., Martineau, A., & Henry, G. F. J.
- Venue
- Transactions of the Royal Society of Tropical Medicine and Hygiene
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-05
- Volume
- 96
- Issue
- 3
- Pages
- 258-265
- ISSN
- 0035-9203 (ISSN-L)
- OpenAlex
- W1978943655
- Cited by
- 89
- References
- 1
- Citations by year
- 2023: 3 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2015: 2 · 2014: 3 · 2013: 3 · 2012: 7
- FWCI
- 6.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Transactions of the Royal Society of Tropical Medicine and Hygiene (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Royal Society
- Royal Society of Tropical Medicine and Hygiene
- South African Medical Research Council
- Abbott Diagnostics
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Keywords
- Plasmodium falciparum, Plasmodium vivax, diagnostic accuracy, detection limit, false positives, OptiMAL, South Africa
- MeSH terms
- Animals, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Parasitology, Plasmodium falciparum, Reagent Kits, Diagnostic, Sensitivity and Specificity, South Africa, Malaria, Falciparum
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