Fluorescent Protein Applications in Microscopy
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Multiple variables can influence the quantitation of fluorophores in fixed tissue samples, so the interpretation of MBP immunofluorescence as a marker of myelination must be considered cautiously.
“Lastly, we have used MBP immunofluorescence as a marker of myelination, however, multiple variables can influence the quantitation of fluorophores in fixed tissue samples (Straight, 2007; Waters, 2009) and this interpretation must be considered cautiously.”
From The role of myelination in measures of white matter integrity: Combination of diffusion tensor imaging and two-photon microscopy of CLARITY intact brains (2017) · cites this paper as Straight, 2007
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Chang, E. H., Argyelan, M., Aggarwal, M., Chandon, T.-S. S., Karlsgodt, K. H., Mori, S., & Malhotra, A. K. (2017) · NeuroImage · doi:10.1016/j.neuroimage.2016.11.068 · Cited by 186 · see citing works
- Authors
- Straight, A. F.
- Affiliations
- Stanford Medicine (US)
- Corresponding author
- Straight, Aaron F.
- ORCID
- Straight, Aaron F.
- Venue
- Methods in Cell Biology
- Publisher
- Elsevier
- Published
- 2007
- Volume
- 114
- Pages
- 93-113
- ISSN
- 0091-679X (ISSN-L)
- OpenAlex
- W1602164555
- Cited by
- 24
- References
- 0
- Citations by year
- 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 3
- FWCI
- 4.65
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in cell biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Keywords
- fluorescent proteins, protein localization, protein dynamics, protein-protein interaction, multi-wavelength imaging, photoactivation, photobleaching, pH-sensitive fluorescent proteins, intracellular environment, live-cell imaging
- MeSH terms
- Image Enhancement, Intracellular Fluid, Microscopy, Fluorescence, Models, Biological, Models, Molecular, Protein Binding, Recombinant Fusion Proteins, Mutagenesis, Site-Directed, Protein Interaction Mapping, Photobleaching, Green Fluorescent Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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