The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Characterization of the Active Intermediate of a GroEL–GroES-Mediated Protein Folding Reaction
Open accessAbstract
Recent studies of GroE-mediated protein folding indicate that substrate proteins are productively released from a cis ternary complex in which the nonnative substrate is sequestered within the GroEL channel underneath GroES. Here, we examine whether protein folding can occur in this space. Stopped-flow fluorescence anisotropy of a pyrene-rhodanese-GroEl complex indicates that addition of GroES and ATP (but not ADP) leads to a rapid change in substrate flexibility at GroEL. Strikingly, when GroES release is blocked by the use of either a nonhydrolyzable ATP analog or a single-ring GroEL mutant, substrates complete folding while remaining associated with chaperonin. We conclude that the cis ternary complex, in the presence of ATP, is the active state intermediate in the GroE-mediated folding reaction: folding is initiated in this state and for some substrates may be completed prior to the timed release of GroES triggered by ATP hydrolysis.
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Cites
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The crystal structure of the bacterial chaperonln GroEL at 2.8 Å
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Self-citationMeasurement of protein-binding phenomena by gel filtration
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Self-citationResidues in chaperonin GroEL required for polypeptide binding and release
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Dynamics of the Chaperonin ATPase Cycle: Implications for Facilitated Protein Folding
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Mechanism of GroEL action: Productive release of polypeptide from a sequestered position under groes
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Cited by
Molecular Chaperones in the Cytosol: from Nascent Chain to Folded Protein
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The crystal structure of the asymmetric GroEL–GroES–(ADP)7 chaperonin complex
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Self-citationA structural change in the kinesin motor protein that drives motility
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Proteome-wide Analysis of Chaperonin-Dependent Protein Folding in Escherichia coli
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STRUCTURE AND FUNCTION IN GroEL-MEDIATED PROTEIN FOLDING
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Metadata
- Authors
- Weissman, J. S., Rye, H. S., Fenton, W. A., Beechem, J. M., & Horwich, A. L.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1996-02
- Volume
- 84
- Issue
- 3
- Pages
- 481-490
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1965255559
- Cited by
- 395
- References
- 24
- FWCI
- 12.00
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Weissman, J. S., Rye, H. S., Fenton, W. A., Beechem, J. M., & Horwich, A. L. (1996). Characterization of the Active Intermediate of a GroEL–GroES-Mediated Protein Folding Reaction. Cell. https://doi.org/10.1016/s0092-8674(00)81293-3
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