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The Polyadenylation Factor CstF-64 Regulates Alternative Processing of IgM Heavy Chain Pre-mRNA during B Cell Differentiation
Open accessAbstract
The switch from membrane-bound to secreted-form IgM that occurs during differentiation of B lymphocytes has long been known to involve regulated processing of the heavy chain pre-mRNA. Here, we show that accumulation of one subunit of an essential polyadenylation factor (CstF-64) is specifically repressed in mouse primary B cells and that overexpression of CstF-64 is sufficient to switch heavy chain expression from membrane-bound (microm) to secreted form (micros). We further show that CstF-64 is limiting for formation of intact CstF, that CstF has a higher affinity for the microm poly(A) site than for the micros site, and that the microm site is stronger in a reconstituted in vitro processing reaction. Our results indicate that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.
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Cited by
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Mayr, C., & Bartel, D. P. (2009) · Cell · doi:10.1016/j.cell.2009.06.016 · Cited by 1,613
HITS-CLIP yields genome-wide insights into brain alternative RNA processing
Licatalosi, D. D., Mele, A., Fak, J. J., Ule, J., Kayikci, M., Chi, S. W., Clark, T. A., Schweitzer, A. C., Blume, J. E., Wang, X., et al. (2008) · Nature · doi:10.1038/nature07488 · Cited by 1,352
“Changes in the accessibility of core (e.g. CPSF and CstF) or auxiliary factors to interact with cis elements surrounding the poly(A) site underlie the regulation of alternative polyadenylation24,25,47,48.”
Sandberg, R., Neilson, J. R., Sarma, A., Sharp, P. A., & Burge, C. B. (2008) · Science · doi:10.1126/science.1155390 · Cited by 1,349
Alternative polyadenylation of mRNA precursors
Tian, B., & Manley, J. L. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.116 · Cited by 1,179
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Metadata
- Authors
- Takagaki, Y., Seipelt, R. L., Peterson, M. L., & Manley, J. L.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1996-11
- Volume
- 87
- Issue
- 5
- Pages
- 941-952
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2027784918
- Cited by
- 425
- References
- 0
- FWCI
- 6.31
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: The Polyadenylation Factor CstF-64 Regulates Alternative Processing of IgM Heavy Chain Pre-mRNA during B Cell Differentiation. https://app.cochat.ai/papers/10.1016/s0092-8674(00)82000-0
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- Takagaki, Y., Seipelt, R. L., Peterson, M. L., & Manley, J. L. (1996). The Polyadenylation Factor CstF-64 Regulates Alternative Processing of IgM Heavy Chain Pre-mRNA during B Cell Differentiation. Cell. https://doi.org/10.1016/s0092-8674(00)82000-0
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