Phospholipase C-γ Is Required for Agonist-Induced Ca2+ Entry
Open accessAbstract
We report here that PLC-gamma isoforms are required for agonist-induced Ca2+ entry (ACE). Overexpressed wild-type PLC-gamma1 or a lipase-inactive mutant PLC-gamma1 each augmented ACE in PC12 cells, while a deletion mutant lacking the region containing the SH3 domain of PLC-gamma1 was ineffective. RNA interference to deplete either PLC-gamma1 or PLC-gamma2 in PC12 and A7r5 cells inhibited ACE. In DT40 B lymphocytes expressing only PLC-gamma2, overexpressed muscarinic M5 receptors (M5R) activated ACE. Using DT40 PLC-gamma2 knockout cells, M5R stimulation of ER Ca2+ store release was unaffected, but ACE was abolished. Normal ACE was restored by transient expression of PLC-gamma2 or a lipase-inactive PLC-gamma2 mutant. The results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
PLCγ2 is a PIP2 metabolising enzyme recruited to the cell membrane upon activation by tyrosine kinase and modulates immune signals impacting cell fate and function.
“The protein product of PLCG2 (PLCγ2) is a PIP2 metabolising enzyme that is recruited to the cell membrane upon activation by tyrosine kinase and modulates immune signals impacting cell fate and function, both in the brain and periphery [12, 13].”
From Heterozygous expression of the Alzheimer’s disease-protective PLCγ2 P522R variant enhances Aβ clearance while preserving synapses (2022) · cites this paper as [13]
No citation lists for this paper
No citation lists for this paper yet — checked again daily.
References 62 · Cited by 176
- Authors
- Patterson, R. L., van Rossum, D. B., Ford, D. L., Hurt, K. J., Bae, S. S., Suh, P.-G., Kurosaki, T., Snyder, S. H., & Gill, D. L.
- Affiliations
- Johns Hopkins University (US)
- University of Maryland, Baltimore (US)
- Pohang University of Science and Technology (KR)
- Kansai Medical University (JP)
- Corresponding author
- Snyder, Solomon H.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2002-11
- Volume
- 111
- Issue
- 4
- Pages
- 529-541
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2139679033
- Cited by
- 176
- References
- 62
- Citations by year
- 2024: 4 · 2022: 3 · 2021: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 6 · 2015: 5 · 2014: 4 · 2013: 1 · 2012: 4
- FWCI
- 8.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 3911 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
- Scholarworks@UNIST (Ulsan National Institute of Science and Technology) (submittedVersion)
- Funders
- National Institutes of Health (NH65090)
- National Heart, Lung, and Blood Institute (R01HL055426, HL55426)
- National Institute of Mental Health (MH-18501)
- National Institute on Drug Abuse (DA 00074)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- phospholipase C-gamma, PLCγ1, PLCγ2, SH3 domain, PC12 cells, A7r5 cells, RNA interference
- MeSH terms
- Animals, Calcium, Catalysis, Cell Line, Transformed, Humans, Isoenzymes, Type C Phospholipases, RNA, Double-Stranded, PC12 Cells, RNA, Small Interfering, Rats, Phospholipase C gamma
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
