The role of calcium signaling in early axonal and dendritic morphogenesis of rat cerebral cortex neurons under non-stimulated growth conditions
Abstract
The effects of depolarizing stimuli on neurite outgrowth have been shown to depend on an influx of extracellular calcium. However, the role of calcium under non-stimulated growth conditions is less well established. Here we investigated the contribution of calcium signaling to early neuronal morphogenesis of rat cerebral cortex neurons at three levels by blocking L-type voltage sensitive calcium channels, by depleting intracellular calcium or by blocking myosin light chain kinase. Detailed quantitative morphological analysis of neurons treated for 1 day revealed that depletion of intracellular calcium strongly decreased the density of filopodia, arrested axonal outgrowth and strongly decreased dendritic branching. Preventing calcium influx through L-type voltage sensitive calcium channels and blocking of myosin light chain kinase activity selectively decreased dendritic branching. Our observations support an essential role for basal intracellular calcium levels in axonal elongation. Furthermore, under non-stimulated conditions calcium entry through L-type voltage sensitive calcium channels and myosin light chain kinase play an important role in dendritic branching.
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Depletion of intracellular calcium in rat cortical neurons arrests axonal growth and reduces dendritic arborization.
“depletion of intracellular calcium in rat cortical neurons arrests axonal growth and reduces dendritic arborization97.”
From CYFIP1 governs the development of cortical axons by modulating calcium availability (2025) · cites this paper as [97]
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References 37 · Cited by 55
- Authors
- Ramakers, G. J. A., Avci, B., van Hulten, P., van Ooyen, A., Pelt, J. V., Pool, C. W., & Lequin, M. B.
- Corresponding author
- Ramakers, G.J.A.
- ORCID
- Pelt, J.van
- Venue
- Developmental Brain Research
- Publisher
- Elsevier BV
- Published
- 2001-02
- Volume
- 126
- Issue
- 2
- Pages
- 163-172
- ISSN
- 0165-3806 (ISSN-L)
- OpenAlex
- W2000012518
- Cited by
- 55
- References
- 37
- Citations by year
- 2025: 2 · 2023: 1 · 2020: 3 · 2019: 3 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 3 · 2012: 7
- FWCI
- 1.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Developmental Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- calcium signaling, neuronal morphogenesis, axon growth, dendritic branching, cortical neurons, L-type calcium channels, intracellular calcium depletion, myosin light chain kinase, filopodia, neurite outgrowth
- MeSH terms
- Animals, Axons, Azepines, Calcium Channel Blockers, Cell Differentiation, Cells, Cultured, Cerebral Cortex, Chelating Agents, Dendrites, Egtazic Acid, Enzyme Inhibitors, Intracellular Fluid, Myosin-Light-Chain Kinase, Naphthalenes, Neurons, Nifedipine, Pseudopodia, Calcium Signaling, Calcium Channels, L-Type, Rats
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