In vivo neutralization of endogenous brain fractalkine increases hippocampal TNFα and 8-isoprostane production induced by intracerebroventricular injection of LPS
Abstract
Fractalkine is a chemokine widely and constitutively expressed in the brain and, as suggested by in vitro studies, it is involved in brain inflammatory responses. In this study, we have investigated the in vivo anti-inflammatory potential of fractalkine in a model of neuroinflammation induced by intracerebroventricular injection of lipopolysaccharide (LPS) in rats. LPS induces a rapid and acute production of the pro-inflammatory cytokine, TNFalpha, in hippocampus and cerebrospinal fluid (CSF), and an increase of 8-isoprostane levels, a marker of oxidative stress, in hippocampus. Although intracerebroventricular injection of fractalkine has no effect on TNFalpha and 8-isoprostane production, neutralization of endogenous fractalkine within the brain with a specific anti-fractalkine antibody potentiates LPS effects. These data emphasize the involvement of constitutive brain fractalkine in the control of inflammatory reaction in CNS.
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Neuronal cells constitutively express CX3CL1, which binds to a G protein-coupled receptor CX3CR1 present in microglia, controlling microglia activation and lowering levels of IL-1β, TNF-α and IL-6.
“Recent evidence has shown that neuronal cells constitutively express CX3CL1, which binds to a G protein-coupled receptor CX3CR1 present in microglia, controlling microglia activation and lowering levels of IL-1β, TNF-α and IL-6 [123,124].”
From Beneficial Effects of Spirulina Consumption on Brain Health (2022) · cites this paper as [124]
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References 42 · Cited by 102
- Authors
- Zujovic, V., Schussler, N., Jourdain, D., Duverger, D., & Taupin, V.
- Affiliations
- Sanofi (France) (FR)
- Corresponding author
- Taupin, Véronique
- ORCID
- Zujovic, Violetta
- Venue
- Journal of Neuroimmunology
- Publisher
- Elsevier BV
- Published
- 2001-04
- Volume
- 115
- Issue
- 1-2
- Pages
- 135-143
- ISSN
- 0165-5728 (ISSN-L)
- OpenAlex
- W1978855889
- Cited by
- 102
- References
- 42
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 6 · 2023: 4 · 2022: 9 · 2021: 4 · 2020: 7 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 4 · 2015: 4 · 2014: 3 · 2013: 2 · 2012: 5
- FWCI
- 2.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroimmunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Keywords
- fractalkine, neuroinflammation, hippocampus, TNF-α, 8-isoprostane, oxidative stress, lipopolysaccharide, chemokine neutralization, cerebrospinal fluid
- MeSH terms
- Animals, Antibodies, Brain, Disease Models, Animal, Encephalitis, Hippocampus, Injections, Intraventricular, Lipopolysaccharides, Male, Membrane Proteins, Tumor Necrosis Factor-alpha, Dinoprost, Rats, Sprague-Dawley, Oxidative Stress, Chemokines, CXC, Chemokines, CX3C, F2-Isoprostanes, Rats, Chemokine CX3CL1
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