Apolipoprotein E is found in astrocytes but not in microglia in the normal mouse brain
Abstract
Apolipoprotein E (apoE) is a known risk factor for Alzheimer's disease, but neither its roles in the pathogenesis nor its exact physiological functions in the brain is known. In order to study the apoE protein in the brains of normal mouse and transgenic mouse models of neurodegeneration, hamster monoclonal antibodies (MAbs) specific to mouse apoE were generated. N- and C-terminal fragments of mouse apoE protein were produced in E. coli as fusion proteins and used to immunize Armenian hamsters. Specificity of the antibodies was established by immunoblotting against sera and brain homogenates of wild type and apoE-deficient mice. MAb 884F11 was found most suitable for immunohistochemistry on 4% PFA-fixed brain tissues. The strongly positive structure in the normal brain was the astrocytes as identified by simultaneous staining for GFAP with lesser and regionally variable diffuse staining of the neuropil. GFAP-positive cells were variable in their content of apoE. ApoE immunoreactivity in the hippocampus and neocortex did not coincide with the tomato lectin binding, indicating that this apolipoprotein is not detectable in the microglial cells of the normal adult mouse brain.
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ApoE was found in astrocytes.
“ApoE was found in astrocytes as well (Fig. 3E), as observed by others (Fujita et al., 1999).”
From Critical Role of Astroglial Apolipoprotein E and Liver X Receptor-α Expression for Microglial Aβ Phagocytosis (2011) · cites this paper as Fujita et al., 1999
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References 45 · Cited by 32
- Authors
- Fujita, S. C., Sakuta, K., Tsuchiya, R., & Hamanaka, H.
- Corresponding author
- Fujita, Shinobu C
- Venue
- Neuroscience Research
- Publisher
- Elsevier BV
- Published
- 1999-11
- Volume
- 35
- Issue
- 2
- Pages
- 123-133
- ISSN
- 0168-0102 (ISSN-L)
- OpenAlex
- W2064422619
- Cited by
- 32
- References
- 45
- Citations by year
- 2019: 1 · 2015: 2 · 2014: 5
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- apolipoprotein E, astrocytes, microglia, ApoE-deficient mice, monoclonal antibodies, glial fibrillary acidic protein, hippocampus, neocortex, tomato lectin, neurodegeneration
- MeSH terms
- Animals, Antibodies, Monoclonal, Antibody Specificity, Apolipoproteins E, Astrocytes, Brain, Glial Fibrillary Acidic Protein, Cricetinae, Immunohistochemistry, Mice, Inbred Strains, Immunoblotting, Microglia, Mice, Knockout, Lectins, Plant Lectins, Mice
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