Estrogen receptor-β regulates transcript levels for oxytocin and arginine vasopressin in the hypothalamic paraventricular nucleus of male mice
Abstract
Estrogen receptor (ER)-beta, unlike ER-alpha, is localized in the hypothalamic paraventricular nucleus (PVN) which also contains neuropeptide synthesizing neurons, such as oxytocin (OT), arginine vasopressin (AVP) and corticotropin-releasing hormone (CRH). Although it is known that some ER-beta containing neurons co-express OT and AVP, but not CRH, in the PVN, it is not yet determined whether ER-beta activation may indeed play a role in estrogenic regulation on syntheses of these neuropeptides. In the present study, we tested this hypothesis by comparing the effects of estrogen on the levels of OT, AVP and CRH messenger RNA (mRNA) between ER-beta knockout (betaERKO) and wild type (WT) control male mice. Mice were gonadectomized and implanted with either a pellet containing estradiol benzoate (2.5-5.0 microg/day) or a placebo pellet for 21 days. In situ hybridization histochemistry revealed that estrogen treatment resulted in a significant increase in OT transcripts (151.6+/-6.0%) and a decrease in AVP transcripts (77.8+/-5.2%) in the PVN of WT mice, compared to the placebo control group. This estrogenic regulation of OT and AVP mRNA levels in the PVN was completely abolished in betaERKO mice. Similar genotype differences in the effects of estrogen on the numbers of OT- and AVP-containing cells were found in immunocytochemical studies performed in a separate set of mice. On the other hand, the expression of CRH mRNA in the PVN was not affected by estrogen treatment in either W…
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Pathways coordinating social recognition are regulated by estrogen, with both ERα and ERβ knockout mice showing social impairments, and ERβ knockout females failing to generate OT or AVP mRNA expression in response to exogenous estrogen.
“Pathways coordinating social recognition are well known to be regulated by estrogen, with both ERα and ERβ knockout mice showing social impairments (130, 131), and ERβ knockout females failing to generate OT or AVP mRNA expression in response to exogenous estrogen administration (132, 133).”
From Endocrine Disruption of Vasopressin Systems and Related Behaviors (2017) · cites this paper as [133]
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- Authors
- Nomura, M., McKenna, E., Korach, K. S., Pfaff, D. W., & Ogawa, S.
- Affiliations
- Rockefeller University (US)
- National Institutes of Health (US)
- Corresponding author
- Ogawa, Sonoko
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 2002-12
- Volume
- 109
- Issue
- 1-2
- Pages
- 84-94
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2037717279
- Cited by
- 167
- References
- 69
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 7 · 2023: 3 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 9 · 2017: 10 · 2016: 5 · 2015: 6 · 2014: 9 · 2013: 9 · 2012: 5
- FWCI
- 16.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service (62147)
- Topics
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- estrogen receptor beta, oxytocin, vasopressin, corticotropin-releasing factor, hypothalamic paraventricular nucleus, neuropeptide gene expression, estradiol benzoate, supraoptic nucleus, medial preoptic area, male mice
- MeSH terms
- Animals, Arginine Vasopressin, Estrogens, Gene Expression Regulation, Male, Oxytocin, Paraventricular Hypothalamic Nucleus, Receptors, Estrogen, RNA, Messenger, In Situ Hybridization, Mice, Knockout, Estrogen Receptor beta, Mice, Rats
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